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Anders Broberg

Anders Broberg
I am professor of natural products chemistry at the Department of Molecular Sciences, and I am conducting research and teach in the field of natural products chemistry. The focus of my research today is to find new antibiotic secondary metabolites from fungi and bacteria.

Teaching

My main teaching responsibility is on the department's course in natural products chemistry (Ke0057, 10 hp), but during the last years also on the basic course in chemistry. The course in natural products chemistry aims at presenting the different groups of natural products with respect to structures, biosynthesis, properties, occurrence, and methods for their analysis. In 2013 I was awarded the ULS' pedagogical price for my work with this course.

Research

Many organisms produce so called secondary metabolites that are used in interactions with other organisms. For example, many plants produce compounds that make them less attractive or even toxic to herbivores and many microorganisms produce compounds of antibiotic type to combat competing microorganisms. Some examples of research questions we try to answer are:

  • Can we find new and efficient antibiotic compounds from cultures of fungi and bacteria?
  • How are selected secondary metabolites biosynthesised, i.e. which metabolic pathways are involved?
  • Which ecological role or function do specific secondary metabolites have?

Information about selected projects can be found here. A brief description of a project aimed at finding new antibiotic compounds is presented here.

Background

  • Professor of natural products chemistry, 23/9, 2014
  • Associate professor of chemistry, 27/10, 2003
  • Researcher, the Department of Chemistry, SLU, 1/2 2000-
  • Post-doctoral fellow, the Carlsberg laboratory, Copenhagen, 1/2 1999 to 31/1 2000.
  • PhD in chemistry, SLU, 31/1 1999.
  • Master in biology, Uppsala university, 1993.

Selected publications

58. Iqbal, M., Broberg, M., Haarith, D., Broberg, A., Bushley, K.E., Brandström Durling, M., Viketoft, M., Funck Jensen, D., Dubey, M., Karlsson, M. 2020. Natural variation of root lesion nematode antagonism in the biocontrol fungus Clonostachys rosea and identification of biocontrol factors through genome-wide association mapping. Evolutionary Applications 00: 1-20. DOI: 10.1111/eva.13001.
 57. Levenfors, J.J., Nord, C., Bjerketorp, J., Ståhlberg, J., Larsson, R, Guss, B., Öberg, B., Broberg, A. 2020. The Journal of Antibiotics, https://doi.org/10.1038/s41429-020-0318-1.

56.

Nord, C., Levenfors, J.J., Bjerketorp, J., Sahlberg, C., Guss, B., Öberg, B., Broberg, A. 2019. Antibacterial isoquinoline alkaloids from the fungus Penicillium spathulatum Em19. Molecules 2019, 24, 4616; doi:10.3390/molecules24244616.

55.

Iqbal, M., Dubey, M., Broberg, A., Viketoft, M., Funck Jensen, D., Karlsson, M. 2019. Deletion of the Non-Ribosomal Peptide Synthetase Gene nps1 in the Fungus Clonostachys rosea Attenuates Antagonism and Biocontrol of Plant Pathogenic Fusarium and Nematodes. Phytopathology, 109(10), 1698-1709.

54.

Azeem, M., Barba-Aliaga, M., Borg-Karlsson, A.K., Terenius, O., Broberg, A., Kuttuva Rajaro, G. 2019. Heterobasidion-growth inhibiting Bacillus subtilis A18 exhibits medium- and age-dependent production of lipopeptides. Microbiological Research 223-225: 129-136.

53.

Fatema, U., Broberg, A., Funck Jensen, D., Karlsson, M., Dubey, M. 2018. Functional analysis of polyketide synthase genes in the biocontrol fungus Clonostachys rosea. Scientific Reports 8:15009, DOI:10.1038/s41598-018-33391-1.

52.

Liu, B., Krishnaswamyreddy, S., Muraleedharan, M.N., Olson, Å., Broberg, A., Ståhlberg, J., Sandgren, M. 2018. Side-by-side biochemical comparison of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare on their activity against crystalline cellulose and glucomannan. PLoS ONE 13(9): e0203430. doi.org/10.1371/journal.pone.0203430.

51.

Kälvö, D., Menkis, A., and Broberg, A. 2018. Secondary metabolites from the root rot biocontrol fungus Phlebiopsis gigantea. Molecules, 23(6), 1417; https://doi.org/10.3390/molecules23061417.

50.

El Hakeem, A., Markovic, D., Broberg, A., Anten, N., Ninkovic, V. 2018. Aboveground mechanical stimuli affect belowground plant-plant communication. PLoS ONE 13(5): e0195646.

49.

Liu, B., Olson, Å., Wu, M., Broberg, A., and Sandgren, M. 2017. Biochemical studies of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare and their roles in lignocellulose degradation. PLoS ONE 12(12): e0189479. doi.org/10.1371/journal.pone.0189479

48.

Bjerketorp, J., Levenfors, J.J., Sahlberg, C., Nord, C., Andersson, P.F., Guss, B., Öberg, B., and Broberg A. 2017. Antibacterial 3,6-disubstituted 4-hydroxy-5,6-dihydro-2H-pyran-2-ones from Serratia plymuthica MF371-2. Journal of Natural Products 80, 2997-3002.

47.

Broberg, A., Bjerketorp, J., Andersson, P., Sahlberg, C., and Levenfors, J. 2017. Labradorins with antibacterial activity produced by Pseudomonas sp. Molecules, 22(7), 1072.

46.

Nord, C., Menkis, A., and Broberg, A. 2015. Cytotoxic Illudane Sesquiterpenes from the Fungus Granulobasidium vellereum (Ellis & Cragin) Jülich. Journal of Natural Products 78, 2559-2564.

 45.

Hansson, D.,Wubshet, S., Olson, Å., Karlsson, M., Staerk, D., and Broberg, A.. 2014. Secondary metabolite comparison of the species within the Heterobasidion annosum s.l. complex. Phytochemistry 108, 243-251.

 44.

Nord, C., Menkis, A., and Broberg, A. 2014. Cytotoxic illudalane sesquiterpenes from the wood-decay fungus Granulobasidium vellereum (Ellis & Craigin) Jûlich. Molecules 19, 14195-14203.

 43.

Nord, C.L., Menkis, A., Lendel, C., Vasaitis, R., and Broberg, A. 2014. Sesquiterpenes from the saprotrophic fungus Granulobasidium vellereum (Ellis & Cragin) Jülich. Phytochemistry 102, 197-204.

 42.

Cleary, M.R., Andersson, P.F., Broberg, A., Elfstrand, M., Daniel, G., and Stenlid, J. 2014. Genotypes of Fraxinus excelsior with different susceptibility to the ash dieback pathogen Hymenoscyphus pseudoalbidus and their response to the phytotoxin viridiol - a metabolomic and microscopic study. Phytochemistry 102, 115-125.

 41.

Hosseini, S., Heyman, F., Olsson, U., Broberg, A., Funck Jensen, D., Karlsson, M. 2014. Zoospore chemotaxis of closely related legume-root infecting Phytophthora species towards host isoflavones. Plant Pathology 63, 708-714.

 40.

Dubey, M., Broberg, A., Funck Jensen, D. and Karlsson, M. 2013. Role of the methylcitrate cycle in growth, antagonism and induction of systemic defence responses in the fungal biocontrol agent Trichoderma atroviride. Microbiology 159, 2492-2500.

 39.

Petersson, E.V., Nahar, N., Dahlin, P., Broberg, A., Åslund-Tröger, R., Dutta, P.C., Jonsson, L., and Sitbon, F. 2013. Conversion of exogenous cholesterol into glycoalkaloids in potato shoots, using two methods for sterol solubilisation. PLoS One 8 (12): e82955. doi:10.1371/journal.pone.0082955.

 38.

 Dubey, M., Broberg, A., Sooriyaarachchi, S., Ubhayasekera, W., Funck Jensen, D. and Karlsson, M. 2013. The glyoxylate cycle is involved in pleotropic phenotypes, antagonism and induction of plant defence responses in the fungal biocontrol agent Trichoderma atroviride. Fungal Genetics and Biology 58-59, 33-41.

 37.

Nord, C.L., Menkis, A., Vasaitis, R. and Broberg, A. 2013. Protoilludane sesquiterpenes from the wood decomposing fungus Granulobasidium vellereum (Ellis & Cragin) Jülich., Phytochemistry 90, 128-134.

36. 

Andersson, P.F., Broberg, A. and Lundberg, D. 2013.  The furanosteroid viridiol. Acta Chrystallogr Sect E., E69, o467-o468.

35.

Andersson, P.F., Bengtsson, S., Cleary, M.R., Stenlid, J. and Broberg, 2013. A. Viridin-like steroids from Hymenoscyphus pseudoalbidus. Phytochemistry 86, 195-200. 

34.

Hansson, D., Menkis, A..,Olson, Å.,,Stenlid, J.. Broberg, A.., Karlsson, M. 2012. Biosynthesis of fomannoxin in the root-rotting pathogen Heterobasidion occidentale. Phytochemistry 84, 31-39.

33. Hansson, D., Menkis, A., Himmelstrand, K., Thelander, M., Olson, Å., Stenlid, J., Karlsson, M. and Broberg, A. 2012. Sesquiterpenes from the conifer root rot pathogen Heterobasidion occidentale. Phytochemistry 82, 158-165.
32. Andersson, P.F., Bengtsson, S., Stenlid, J. and Broberg, A. 2012. B-norsteroids from Hymenoscyphus pseudoalbidus. Molecules 17, 7769-7781.
31. Olson, Å., Aerts, A., Asiegbu, F., Belbahri, L., Bouzid, O., Broberg, A., Canbäck, B., Coutinho, P.M., Cullen, D., Dalman, K., Deflorio, G., van Diepen, L.T.A, Dunand, C., Duplessis, S., Durling, M., Gonthier, P., Grimwood, J., Fossdal, C.G., Hansson, D., Henrissat, B., Hietala, A., Himmelstrand, K., Hoffmeister, D., Högberg, N., James, T.Y., Karlsson, M., Kohler, A., Kües, U., Lee, Y.-H., Lin, Y.-C., Lind, M., Lindquist, E., Lombard, V., Lucas, S., Lundén, K., Morin, E., Murat, C., Park, J., Raffaello, T., Rouzé, P., Salamov, A., Schmutz, J., Solheim, H., Ståhlberg, J., Vélëz, H., de Vries, R.-P., Wiebenga, A., Woodward, S., Yakovlev, I., Garbelotto, M., Martin, F., Grigoriev, I.V., Stenlid, J. 2012. Insight into trade-off between wood decay and parasitism from the genome of a fungal forest pathogen.New Phytologist 194, 1001-1013.
30. Andersson, P.F., Levenfors, J. and Broberg, A. 2012. Metabolites from Pseudomonas brassicacearum with activity against the pink snow mould causing pathogen Microdochium nivale. BioControl 57, 463-469.
29. Lind, H., Broberg, A., Jacobsson, K., Jonsson, H. and Schnürer, J. 2011. Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria. International Journal of Microbiology. Published on-line 23/1-2011
28. Zhokhov, S., Broberg, A., Kenne, L., Jastrebova, J. 2010. Content of antioxidant hydroquinones substituted by β-1,6-linked oligosaccharides in wheat milled fractions, flours and breads. Food Chemistry 121, 645-652.
27. Andersson, P.F., Johansson, S., Stenlid, J., Broberg, A. 2010. Isolation, identification and necrotic activity of viridiol from Chalara fraxinea, the fungus responsible for dieback of ash. Forest Pathology 40, 43-46.
26. Zhokhov, S., Jastrebova, J., Kenne, L., Broberg, A. 2009. Antioxidant hydroquinones substituted by beta-1,6-linked oligosaccharides in wheat germ. Journal of Natural Products 72, 656-661.
25. Leong, S.-L., Schnürer, J., Broberg, A. 2008. Verrucine F – a new quinazoline from Penicillium verrucosum. Journal of Natural Products 71, 1455-1457.
24. Broberg, A., Jacobsson, K., Ström, K., Schnürer, J. 2007. Metabolite profiles of lactic acid bacteria in grass silage. Applied and Environmental Microbiology 73, 5547-5552.
23. Broberg, A. 2007.High-Performance Liquid Chromatography/Electrospray Ionization Ion-Trap Mass Spectrometry for Analysis of Oligosaccharides Derivatized by Reductive Amination and N,N-Dimethylation. Carbohydrate Research 342, 1462-1469.
22. Lind, H., Sjögren, J., A., Gohil, S., Kenne, L., Schnürer, J., Broberg, A. 2007. Antifungal compounds from cultures of dairy propionibacteria type strains. FEMS Microbiology Letters 271, 310-315.
21. Pohanka, A., Menkis, A., Levenfors, J., Broberg, A. 2006. Low-Abundance Kutznerides from Kutzneria sp. 744. Journal of Natural Products 69(12), 1776-1781.
20. Pohanka, A., Levenfors, J., Broberg, A. 2006. Antimicrobial Dialkylresorcinols from Pseudomonas sp. Ki19. Journal of Natural Products 69(4), 654-657.
19. Broberg, A., Menkis, A., Vasiliauskas, R. 2006. Kutznerides 1-4, Depsipeptides from the Actinomycete Kutzneria sp. 744 Inhabiting Mycorrhizal Roots of Picea abies Seedlings. Journal of Natural Products 69(1), 97-102.
18. Pohanka, A., Broberg, A., Johansson, M., Kenne, L., and Levenfors, J. 2005. Pseudotrienic acid A and B, two novel bioactive metabolites from Pseudomonas sp. MF381-IODS. Journal of Natural Products 68, 1380-1385.
17. de Boer, H.J., de Boer-Kool, A., Broberg, A., Mziray, W.R., Hedberg, I, Levenfors, J.J. 2005. Anti-fungal and anti-bacterial activity of some herbal remedies from Tanzania. Journal of Ethnopharmacology 96, 461-469.
16. Omer Z.S., Tombolini, R., Broberg, A. and Gerhardson, B. 2004. Indole-3-acetic acid production in pink-pigmented facultative methylotrophic bacterial culture. Plant growh regulation 43, 93-96.
15. Pohanka, A., Capieau, K., Broberg, A., Stenlid, J., Stenström, E. and Kenne, L. 2004. Enniatins produced by the Fusarium sp. strain F31, an inhibitor of Botrytis cinirea spore germination. Journal of Natural Products 67, 851-857.
14. Nerinckx, W., Broberg, A., Duus, J.Ø., Ntarima, P., Parolis, L.A.S., Parolis, H. and Claeyssens, M. 2004. Hydrolysis of Nothogenia erinacea xylan by xylanases from Families 10 and 11. Carbohydrate Research 339 (6), 1047-1060.
13. Sjögren, J., Magnusson, J., Broberg, A., Schnürer, J. and Kenne, L. 2003. Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14. Applied and Environmental Microbiology 69, 7554-7557.
12. Fredlund E., Broberg, A., Boysen, M., Kenne, L. and Schnürer, J. 2004. Metabolite profiles of the biocontrol yeast Pichia anomala J121 grown under oxygen limitation. Applied Microbiology and Biotechnology 64, 403-409.
11. Iakovlev, A., Broberg, A. and Stenlid, J. 2003. Fungal modification of the hydroxyl radical detector coumarin-3-carboxylic acid. FEMS Microbiology Ecology 46, 197-202.
10. Ström, K.S., Sjögren, J., Broberg, A. and Schnürer, J. 2002. Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro), cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Applied and Environmental Microbiology 68, 4322-4327.
9. Molinaro, A., De Castro, C., Lanzetta, R., Parrilli, M., Petersen, B.O., Broberg A. and Duus, J.Ø. 2002. NMR and MS evidences for a random assembled O-polysaccharide chain structure in the LPS of the bacterium Xanthomonas campestris pv. vitians: A case of unsystematic biosynthetic polymerisation. European Journal of Biochemistry 269, 4185-4193.
8. Broberg, S., Broberg, A. and Duus, J.Ø. 2000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on oligosaccharides derivatized by reductive amination and N,N-dimethylation. Rapid Communications in Mass Spectrometry 14, 1801-1805.
7. Ferré, H., Broberg, A., Duus, J.Ø. and Thomsen, K.K.T. 2000. A novel type of arabinoxylan arabinofuranohydrolase isolated from from germinated barley. Analysis of substrate preference and specificity by nano-probe NMR. European Journal of Biochemistry 267, 6633-6641.
6. Broberg, A., Thomsen, K.K.T. and Duus, J.Ø. 2000. Application of nano-probe NMR for structure determination of low nanomole amounts of arabinoxylan oligosaccharides fractionated by analytical HPAEC-PAD. Carbohydrate Research 328, 375-382.
5. Broberg, A. and Kenne, L. 2000. Use of high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy for in-situ studies of low-molecular mass compounds in red algae. Analytical Biochemistry 284, 367-374.
4. Broberg, A., Kenne, L. and Pedersén, M. 1999. Analysis of 1,5-anhydro-D-fructose, microthecin, and 4-deoxy-glycero-hexo-2,3-diulose in algae using gas chromatography-mass spectrometry in selected ion monitoring mode. Analytical Biochemistry 268, 35-42.
3. Broberg, A., Kenne, L. and Pedersén, M. 1998. In-situ identification of major metabolites in the red alga Gracilariopsis lemaneiformis using high-resolution magic-angle spinning nuclear magnetic resonance spectroscopy. Planta 206, 300-307.
2. Broberg, A., Kenne, L. and Pedersén, M. 1998. Formation of 4-deoxy-glycero-hexo-2,3-diulo-furanose from microthecin. Carbohydrate Research 306, 171-175.
1. Broberg, A., Kenne, L. and Pedersén, M. 1996. Presence of micothecin in the red alga Gracilariopsis lemaneiformis and its formation from 1,5-anhydro-D-fructose. Phytochemistry 41, 151-154.

Contact
Professor at the Department of Molecular Sciences; Organisk kemi
Telephone: +4618672217
Postal address:
Institutionen för molekylära vetenskaper
Box 7015
750 07 Uppsala
Visiting address: Almas Allé 5, BioCentrum, Ultuna, Uppsala