Compound-Specific Stable Isotope Analyses (CSIA)

Last changed: 02 February 2018

15N in amino acids

Samples are derivatized with TMS according to Gullberg et al 2004. The resulting TMS derivatives are analyzed on a Gas Chromatograph - Isotope Ratio Mass Spectrometer - Mass Spectrometer (GC-IRMS-MS). The instrument setup consists of a gas chromatograph (Trace GC Ultra) connected to both a continuous flow isotope ratio mass spectrometer (DeltaV) through GC IsoLink and an organic mass spectrometer (ISQ LT), all from Thermo Fisher Scientific, Bremen, Germany. Isotopic signatures of the samples are calibrated using inhouse laboratory amino acid standards, which have been standardised against four certified standards:IAEA-600, IAEA-N-2, USGS40, and USGS41

J Gullberg, P Jonsson, A Nordström, M Sjöström, T Moritz, Analytical biochemistry 331 (2004), 283-295: Design of experiments: an efficient strategy to identify factors influencing extraction and derivatization of Arabidopsis thaliana samples in metabolomic studies with gas chromatography/mass spectrometry

13C in amino acids

Samples are derivatized with TMS according to Gullberg et al 2004. The resulting TMS derivatives are analyzed on a Gas Chromatograph - Isotope Ratio Mass Spectrometer - Mass Spectrometer (GC-IRMS-MS). The instrument setup consists of a gas chromatograph (Trace GC Ultra) connected to both a continuous flow isotope ratio mass spectrometer (DeltaV) through GC IsoLink and an organic mass spectrometer (ISQ LT), all from Thermo Fisher Scientific, Bremen, Germany. Isotopic signatures of the samples are calibrated using inhouse laboratory amino acid standards, which have been standardised against four certified standards: IAEA-600, IAEA-CH-6, USGS40, and USGS41

J Gullberg, P Jonsson, A Nordström, M Sjöström, T Moritz, Analytical biochemistry 331 (2004), 283-295: Design of experiments: an efficient strategy to identify factors influencing extraction and derivatization of Arabidopsis thaliana samples in metabolomic studies with gas chromatography/mass spectrometry

13C in Phospholipid-derived fatty acids (PLFAs)

Coming in 2018.


Contact

Mats Öquist, Associate Professor and Laboratory Director
Department of Forest Ecology and Management, SLU
mats.oquist@slu.se, +4690-786 8525, +46(0)70-554 0361

Jenny Ekman, Project coordinator
Department of Forest Ecology and Management, SLU
jenny.ekman@slu.se, +46(0)72-538 4633