Our main model organisms are Arabidopsis thaliana and Norway spruce (Picea abies). Combination of an angiosperm and a gymnosperm models enables evolutionary interpretation of our findings.
A gradient of programmed cell death along apical-basal axis of a Norway spruce embryo (from left to right), which is composed of a living embryonal mass (EM) and several layers of suspensor cells with increased degree of disassembly towards basal pole of the embryo. This model system provides a powerful paradigm for studying mechanisms of developmental cell death.
Reza SH, Delhomme N, Street NR, Ramachandran P, Dalman K, Nilsson O, Minina EA, Bozhkov PV (2018) Transcriptome analysis of embryonic domains in Norway spruce reveals potential regulators of suspensor cell death. PLoS One 13: e0192945
Smertenko A and Bozhkov PV (2014) Somatic embryogenesis: balancing life and death processes during apical-basal patterning. J Exp Bot. 65: 1343-1360
Van Doorn W, Beers E, Dangl J, Franklin-Tong V, Gallois P, Hara-Nishimura I, Jones A, Kawai-Yamada M, Lam E, Mundy J, Mur L, Petersen M, Smertenko A, Taliansky M, Van Breusegem F, Wolpert T, Woltering E, Zhivotovsky B, Bozhkov PV. (2011) Morphological classification of plant cell deaths. Cell Death and Differentiation 18: 1241-1246.
M.F.Suarez and P. Bozhkov (Editors). Plant Embryogenesis Methods and Protocols. Series: Methods in Molecular Biology, Vol. 427 Humana Press. 2008. ISBN: 978-1-58829-931-4.
Bozhkov P (2006) Spruce embryogenesis – a model for developmental cell death in plants. In: (Ya. Blume, D. Durzan & P. Smertenko, eds) Cell Biology and Instrumentation: UV radiation, Nitric Oxide and Cell Death in Plants. IOS Press NATO Science Series, Vol. 371, pp. 276-280.
Bozhkov PV, Suarez MF and Filonova LH. (2005) Programmed cell death in plant embryogenesis. Current Topics in Developmental Biology 67: 135-179.
Smertenko AP, Bozhkov PV, Filonova LH, von Arnold S, and Hussey PJ. (2003) Reorganisation of the cytoskeleton during developmental programmed cell death in Picea abies embryos. The Plant Journal 33: 813-824.
Filonova LH, von Arnold S, Daniel G and Bozhkov PV (2002) Programmed cell death eliminates all but one embryo in a polyembryonic plant seed. Cell Death and Differentiation 9: 1057-1062.
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