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BI1344

Applied Plant Biotechnology

The course aims at giving theoretical and practical knowledge in plant tissue culture, especially shoot and root formation, genetic transformation and different molecular biological methods that are used for improvement of agricultural and horticultural crops. Case studies provide the students possibilities to solve various problems using the knowledge they have learnt from the course. After having gone through the course, the students should have better theoretical and practical knowledge in tissue culture and genetic transformation, and have better understanding in using different molecular biological methods and applications of biotechnology in plant breeding.

Dear all,

You are warmly welcome to attend the course. The time schedule for the course is already avaible on this website and minor modifications may happen before the course start. The course room in Canvas is also open. Once the student list is available, you will be invited to the class room.

Due to Covid-19, the lectures will be held online via zoom. Depending on the covid-19 situation, the seminars maybe held in a class room in Alnarp. More updated information will come later on after the course start.

You are welcome to contact me if you have any specific questions related to the course.

Best wishes,

Li-Hua Zhu

The course evaluation is open

Please fill in the evaluation form

You have until 2021-11-15

Additional course evaluations for BI1344

Academic year 2021/2022

Applied Plant Biotechnology (BI1344-M1129) 2021-08-30 - 2021-11-01

Academic year 2020/2021

Applied Plant Biotechnology (BI1344-10158) 2020-08-31 - 2020-11-01

Academic year 2019/2020

Applied Plant Biotechnology (BI1344-10081) 2019-09-02 - 2019-10-31

BI1344 Applied Plant Biotechnology, 15.0 Credits

Applied Plant Biotechnology

Syllabus approved

2018-11-21

Subjects

Biology Horticultural science

Education cycle

Master’s level

Advanced study in the main field

Second cycle, only first-cycle courses as entry requirements(A1N)

Grading scale

5:Pass with Distinction, 4:Pass with Credit, 3:Pass, U:Fail The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.

Language

English

Prior knowledge

Knowledge equivalent to 120 credits, of which at least 60 credits in Biology or 60 credits in Horticultural Science, and English 6.

Objectives

On completion of the course, students will be able to:
- analyse factors affecting micropropagation and perform tissue culture independently
- describe different genetic transformation methods and techniques used in molecular biology
- independently design a research project using biotechnological approaches.

Content

The course comprises:
- theory of tissue culture, in particular in vitro shoot and root culture, genetic transformation, gene cloning and various techniques in molecular biology
- establishment of new in vitro cultures, meristem culture and in vitro rooting, genetic transformation via Agrobacterium-mediated gene transfer, DNA-isolation and analyses of gene expression and PCR
- demonstrations of transformation methods such as particle bombardment and electroporation
- project design focusing on specific problems, e.g. root formation, disease resistance and quality aspects to illustrate potential solutions that biotechnology can provide.
Scheduled practicals, seminars and study visits are compulsory.

Formats and requirements for examination

The examination consists of written and oral presentation of reports. To pass the course, approved participation in all course components, approved reports and approved oral presentations are required.
  • If the student fails a test, the examiner may give the student a supplementary assignment, provided this is possible and there is reason to do so.
  • If the student has been granted special educational support because of a disability, the examiner has the right to offer the student an adapted test, or provide an alternative assessment.
  • If changes are made to this course syllabus, or if the course is closed, SLU shall decide on transitional rules for examination of students admitted under this syllabus but who have not yet passed the course.
  • For the examination of a degree project (independent project), the examiner may also allow the student to add supplemental information after the deadline. For more information on this, please refer to the regulations for education at Bachelor's and Master's level.

Other information

  • The right to take part in teaching and/or supervision only applies to the course date to which the student has been admitted and registered on.
  • If there are special reasons, the student may take part in course components that require compulsory attendance at a later date. For more information on this, please refer to the regulations for education at Bachelor's and Master's level.

Responsible department

Department of Plant Breeding

Further information

Determined by: Programnämnden för utbildning inom landskap och trädgård (PN - LT)
Biology field: Botanik (växtbiologi)
Replaces: BI0911, BI1061

Applied Plant Biotechnology, BI1344, 15hp

Objectives

Criteria

Grade 3

Grade 4

Grade 5

1. Analyze factors affecting micropropagation and perform tissue culture independently

analyse factors affecting micropropagation and perform tissue culture independently

2. Describe different genetic transformation methods and techniques used in molecular biology and genetic engineering

In the case study, use and describe briefly different transformation methods and techniques used in molecular biology and genetic engineering.

Besides these in grade 3, discuss advantages and disadvantages of important modern techniques used in genetic improvement of plant properties.

3. Independently design a research project (case study) using molecular and biotechnological approaches.

Independently design a research project (case study) using molecular and biotechnological approaches.

In the research plan of the case study, the research goals are clear, experimental designs are logical, methods are feasible and expected results are clearly stated.

In the research plan of the case study, the research goals are clear, experimental designs are logical, methods are feasible, expected results are clearly stated, and potential problems and possible solutions are stated and proposed.

The above criteria should be clearly shown in 1) written report of the case study and 2) oral presentations on the three seminars. The quality of presentations and questions arose to presenters by opponents as well as active participation in discussions during seminar will be taken into consideration when grading!

Course credits:

The course credits will be based on the results of seminar on literature study, performance and presentation of laboratory practices, seminar and written report of the case study. The final grading will consist different parts of the course according to the table below:

Presentation of literature study

Presentation of case study

Case study rapport

Lab work

Other compulsory activities

10%

20%

40%

20%

10%

Pass (3): Fulfil all criteria at grade 3, participation in course compulsory activities and approved report for the case study handed in within the deadline.

Pass with merit (4): fulfil at least 60 % of the criteria at grade 4, participation in course compulsory activities and approved report for the case study handed in within the deadline

Pass with distinction: (5): fulfil at least 60% of the criteria at grade 5, participation in course compulsory activities and approved report for the case study handed in within the deadline

1) Regarding course literature
Comments: There is no fixed literature for this course. The relevant articles will be loaded on the course room in Canvas.

Course facts

The course is offered as an independent course: Yes The course is offered as a programme course: Horticultural Science - Master´s Programme Horticultural Science Programme Tuition fee: Tuition fee only for non-EU/EEA/Switzerland citizens: 38054 SEK Cycle: Master’s level
Subject: Biology Horticultural science
Course code: BI1344 Application code: SLU-10129 Distance course: No Language: English Responsible department: Department of Plant Breeding Pace: 100%