PNS0138 Confocal Microscopy, 5.0 Credits
Pass / Failed
The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.
The practical part of the course is primarily intended for PhD students within the SLU Graduate School Organism Biology, but will be open for all interested PhD students and researchers if space allows. All lectures will be carried out as open events, no registration is required to attend the theoretical part of the course.
After the course students will be able:
- to choose the optimal microscopy method for a given task
- to plan an experiment with controls which will not rely on "seeing is believing" concept
- to perform confocal microscopy
- to use ZEN and ImageJ software for image analysis
- produce publication-quality images
The course includes a solid theoretical background in confocal microscopy in form of lectures and seminars. Students will have an opportunity to learn from experts who are running microscopy facilities at SciLifeLab and from researchers who are developing cutting edge microscopy technologies. All students will get hands-on experience with confocal microscope and will have an introduction to two-photon, SIM and LightSheet microscopes. The course will provide opportunities for students to visit microscopy facilities available in Uppsala and also learn about other technologies available in Sweden and abroad. Students will be introduced to a set of available software for image analysis including Carl Zeiss ZEN and open source ImageJ.
Formats and requirements for examination
A student should attend at least 80% of lectures and seminars and complete the practical part of the course. During the practical part students will work in small groups. An experiment for each group will be planned in advance to introduce students to advantages and disadvantages of different confocal microscopes. Each student will have to write a brief report in form of a materials and methods chapter plus a figure, prepared as it would be for a publication. In addition, each group will give an oral presentation at the final seminar and discuss with other groups.
The course is organized by Adrian Dauphinee and Stefanie Rosa on behalf of the SLU graduate school Organism Biology. Maximum 15 students per course occasion.
Department of Plant Biology