Confocal Microscopy
Syllabus and other information
Syllabus
PNS0138 Confocal Microscopy, 5.0 Credits
Subjects
BiologyEducation cycle
Postgraduate levelGrading scale
Language
EnglishPrior knowledge
The practical part of the course is primarily intended for PhD students within the SLU Graduate School Organism Biology, but will be open for all interested PhD students and researchers if space allows. All lectures will be carried out as open events, no registration is required to attend the theoretical part of the course.Objectives
After the course students will be able:
to choose the optimal microscopy method for a given task
to plan an experiment with controls which will not rely on "seeing is believing" concept
to perform confocal microscopy
to use ZEN and ImageJ software for image analysis
produce publication-quality images
Content
The course includes a solid theoretical background in confocal microscopy in form of lectures and seminars. Students will have an opportunity to learn from experts who are running microscopy facilities at SciLifeLab and from researchers who are developing cutting edge microscopy technologies. All students will get hands-on experience with confocal microscope and will have an introduction to two-photon, SIM and LightSheet microscopes. The course will provide opportunities for students to visit microscopy facilities available in Uppsala and also learn about other technologies available in Sweden and abroad. Students will be introduced to a set of available software for image analysis including Carl Zeiss ZEN and open source ImageJ.
Additional information
The course is organized by Adrian Dauphinee and Stefanie Rosa on behalf of the SLU graduate schools Organism Biology and Focus on food and biomaterials. Maximum 15 students per course occasion.Responsible department
Department of Plant Biology