PNS0232 Hands on Metabolomics, 5.0 Credits
Subjects Chemistry Biology
No Level Indicated
Pass / Failed
The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.
Undergraduate degree in agronomy, biology and chemistry or related areas. A basic understanding of Matlab and/or R programing languages is recommended.
Metabolomics definition and application: Metabolomics is the systematic study of the unique chemical fingerprints that specific cellular processes leave behind. It is widely applied in several areas of biological research including agronomy, veterinary medicine, nutrition, cell biology and medicine. Recent progress in high-throughput analytical technologies allows the simultaneous analysis of a large number of metabolites in different biofluids and tissues using advanced analytical approaches i.e. NMR and mass spectrometry methods. These analyses can potentially reveal a complex metabolic fingerprint characteristic of a given physiological status e.g. health and disease, dietary exposure, or a characteristic quality.
The course aims at hand-on experiment approach in metabolomics and provides the students with basic understanding of advantages and disadvantages of different analytical strategies in metabolomics, data processing workflow, statistical analysis and the interpretation of metabolic alteration.
After completing the course the students shall
• Be able to describe the work flow in metabolomics analysis
• Be able to describe advantages and disadvantages of different analytical methods used in metabolomics
• Be able to describe the work flow for data processing in metabolomics
• Be able to describe different statistical methods used in metabolomics
• Know availability of different open access data bases in the field of metabolomics
The course starts with introductory lectures about the principle of NMR and mass spectrometry, multivariate statistics, and application of NMR and mass spectrometry in metabolomics analysis. Subsequently, students are provided with simple NMR and mass spectrometry data during workshops where together with instructors they practice step by step converting spectral data to variables. The students should then write a short report about the application.
Formats and requirements for examination
Attendance in lectures and writing the final report
Course organizers: Ali Moazzami, Hanna Eriksson Rönisch
Research school: FFB (Focus on Food and Biomaterials)
Department of Molecular Sciences