Syllabus

PVS0137 Genome Analysis, 10.0 Credits

Subjects

Education cycle

Grading scale

Language

Prior knowledge

Objectives

The course is expected to provide advanced knowledge concerning methodologies for studies on prokaryotic and eukaryotic genomes, as well as advanced knowledge of genome organization and genome evolution. The contents of the course include genome analysis of domestic animals. Methodological and theoretical aspects of the course are applicable to many different organisms.

After completion of the course, the student should be able to:

• comprehensively describe organization and evolution of different eucaryotic genomes and genes, as well as gene regulation.

• explain basic statistics relevant to genome analysis

• illustrate and distinguish between the types of genetic recombination and recite some of its applications in genome analysis

• give a comprehensive account of different types of genetic variation and state of the art methodologies for analysis of genetic variation

• describe a few methods for large scale gene expression analysis

• explain the principles of genome mapping (including whole genome sequencing) and how different mapping techniques are used to identify genes underlying disease or other important phenotypic traits in animals

• review different gene mapping methods including mapping of quantitative traits (QTL-mapping)

• describe epigenetic signatures and methods for their analysis

• summarize the key features of molecular phylogenomics and evolutionary analysis

• describe different approaches for functional analysis of genes and DNA sequence variation

• integrate knowledge in genetics, molecular genetics and biotechnology to solve complex problems of relevance for genome analysis

• independently search for, summarize, interprete and critically assess scientific articles in genetics, molecular genetics and genomics

• summarize the importance of and how bioinformatics can be used in genome analysis

Content

The contents of the course are to a large extent built upon recent advances within the field of genome analyses in humans, animals and plants. A large part of the course is applicable also in e.g. human genetics. Genome science is evolving rapidly and the course is based on recent research developments in methodologies and theories.

The following group discussions or computer-based exercises are included in the course:

• Genetic diversity and polymorphisms

• Molecular phylogenomics (computer exercise)

• Structural and comparative genomics

• Gene mapping and functional genomics

• Genome-wide association analysis (GWAS) (computer exercise)

• Genome-wide association and quantitative trait loci (QTL) analysis

• High throughput genome sequencing

• High throughput genome sequencing (computer exercise)

• Epigenetics and epigenomics

• Genome editing and transgenic animals

Additional information

Responsible department

Department of Animal Breeding and Genetics