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PVS0150

Flow cytometry, cell analysis and sorting

The first week of the course contains lectures before lunch each day, while the afternoons are devoted to practical laboratory work. If possible, a study visit to the BioVis Facility at Uppsala University is arranged. The final two weeks of the course, the students perform individual work with applying flow cytometry to their own projects.

Syllabus

PVS0150 Flow cytometry, cell analysis and sorting, 5.0 Credits

Subjects

Clinical Science

Education cycle

Postgraduate level

Grading scale

Pass / Failed The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.

Language

English

Prior knowledge

Admitted to a postgraduate program in animal science, biology, veterinary medicine, food science, nutrition, nursing, or related subjects, or to a residency program in veterinary science

Objectives

After completing the course the student shall be able to describe the basic components of flow cytometers and confocal laser scanning microscopes and how they work together. The student shall be able to interpret data output from the specialized software for flow cytometry. The student shall be able to describe the principles of cell sorting. The student shall be able do design a basic panel of fluorophores for use in flow cytometry experiments. The student shall be able to apply flow cytometry to his/her research subject.

Content

The first week of the course contains lectures before lunch each day, while the afternoons are devoted to practical laboratory work. If possible, a study visit to the BioVis Facility at Uppsala University is arranged. The final two weeks of the course, the students perform individual work with applying flow cytometry to their own projects.

Formats and requirements for examination

Written Exam+ Approved course report

Additional information

Organised within the Cells for Life Infrastructure

Responsible department

Department of Clinical Sciences

Course facts

Subject: Clinical Science
Course code: PVS0150 Location: Uppsala Distance course: No Language: English Responsible department: Department of Clinical Sciences Pace: 100%