PVS0162 Infection and Reproduction, 3.0 Credits
Pass / Failed
The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.
Admitted to a postgraduate program in animal science, biology, veterinary medicine, food science, animal nutrition, veterinary nursing, or related subjects, or to a residency program in veterinary science
After completing the course the student shall:
- be able to describe different methods for evaluation of sperm
quality, such as membrane integrity, mitochondrial membrane potential and chromatin integrity.
- be able to use the results from the methods decribed above to
make a fertility prediction.
- be able to display basic knowledge of the methodology and
reagents needed for cell culture, virus isolation and virus detection by immunofluorescence.
- describe the basic theory behind immunostaining of cellular
proteins or viruses
- be able to conduct a basic cell culture, virus isolation and
immunofluorescent microscopy analysis
The course will contain lectures and laborations focussing on in vitro
and cellular aspects of infection and reproduction. Techniques
involved and taught to the students will include cell culture, immunological techniques, fluorescence microscopy and flow
cytometry. Laborations will contain cell culture, infections of cell lines with viruses, immunostaining and visualisation by fluorescent microscopy. Laborations on different aspects of sperm quality will also be performed. These will include evaluating membrane integrity
using SYBR‐14/PI staining followed by flow cytometry, performing the sperm chromatin structure assay (SCSA) using acridine orange staining evalauted by flow cytometry, and determining the mitochondrial membrane potential of the spermatozoa using the stain JC-1. The course will contain 16 h lectures, 40 h laborations and 24 h own studies.
Formats and requirements for examination
Written exam. To pass the course the student should score a sufficient number of points in the written exam. The questions will be formulated based on the objectives of the course.
Organised within the Cells for Life Infrastructure. Participation through video-link or studies from distance are not possible, since this is a laboration‐intensive course.
For application: https://www.slu.se/gs-vmas-courses