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PVS0162
Infection and Reproduction
The course will contain lectures and laborations focussing on in vitro
and cellular aspects of infection and reproduction. Techniques
involved and taught to the students will include cell culture, immunological techniques, fluorescence microscopy and flow
cytometry. Laborations will contain cell culture, infections of cell lines with viruses, immunostaining and visualisation by fluorescent microscopy. Laborations on different aspects of sperm quality will also be performed. These will include evaluating membrane integrity
using SYBR‐14/PI staining followed by flow cytometry, performing the sperm chromatin structure assay (SCSA) using acridine orange staining evalauted by flow cytometry, and determining the mitochondrial membrane potential of the spermatozoa using the stain JC-1. The course will contain 16 h lectures, 40 h laborations and 24 h own studies.
and cellular aspects of infection and reproduction. Techniques
involved and taught to the students will include cell culture, immunological techniques, fluorescence microscopy and flow
cytometry. Laborations will contain cell culture, infections of cell lines with viruses, immunostaining and visualisation by fluorescent microscopy. Laborations on different aspects of sperm quality will also be performed. These will include evaluating membrane integrity
using SYBR‐14/PI staining followed by flow cytometry, performing the sperm chromatin structure assay (SCSA) using acridine orange staining evalauted by flow cytometry, and determining the mitochondrial membrane potential of the spermatozoa using the stain JC-1. The course will contain 16 h lectures, 40 h laborations and 24 h own studies.
Syllabus and other information
Syllabus
PVS0162 Infection and Reproduction, 3.0 Credits
Subjects
Veterinary MedicineEducation cycle
Postgraduate levelGrading scale
Pass / Failed
Prior knowledge
Admitted to a postgraduate program in animal science, biology, veterinary medicine, food science, animal nutrition, veterinary nursing, or related subjects, or to a residency program in veterinary scienceObjectives
After completing the course the student shall: - be able to describe different methods for evaluation of sperm quality, such as membrane integrity, mitochondrial membrane potential and chromatin integrity. - be able to use the results from the methods decribed above to make a fertility prediction. - be able to display basic knowledge of the methodology and reagents needed for cell culture, virus isolation and virus detection by immunofluorescence. - describe the basic theory behind immunostaining of cellular proteins or viruses - be able to conduct a basic cell culture, virus isolation and immunofluorescent microscopy analysisContent
The course will contain lectures and laborations focussing on in vitro and cellular aspects of infection and reproduction. Techniques involved and taught to the students will include cell culture, immunological techniques, fluorescence microscopy and flow cytometry. Laborations will contain cell culture, infections of cell lines with viruses, immunostaining and visualisation by fluorescent microscopy. Laborations on different aspects of sperm quality will also be performed. These will include evaluating membrane integrity using SYBR‐14/PI staining followed by flow cytometry, performing the sperm chromatin structure assay (SCSA) using acridine orange staining evalauted by flow cytometry, and determining the mitochondrial membrane potential of the spermatozoa using the stain JC-1. The course will contain 16 h lectures, 40 h laborations and 24 h own studies.Additional information
Organised within the Cells for Life Infrastructure. Participation through video-link or studies from distance are not possible, since this is a laboration‐intensive course.For application: https://www.slu.se/gs-vmas-courses