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Genetics underpinning dynamic laryngeal collapse

Last changed: 17 November 2020
Järvsöfaks

Dynamic laryngeal collapse associated with poll flexion is the most common disorder of the upper respiratory tract in the Norwegian‐Swedish Coldblooded Trotter. The disorder, which has also been diagnosed in other breeds of trotters and gaited horses, appears to be related to anatomic phenotypes and only occurs during poll flexion when the horse is exercised ‘on the bit’.

The objective of the current study was to identify genomic regions associated with dynamic laryngeal collapse (DLC) in the Norwegian‐Swedish Coldblooded Trotter (NSCT) by combining a rigorous phenotyping protocol with genomic data from a high‐density equine genotyping array.

Study design

Prospective case/control study.

Methods

High‐speed treadmill endoscopy was used to phenotype horses (n = 61) for DLC, distinguishing between cases and controls. Genome‐wide association (GWA) analysis of DLC status was then performed using a principal component approach (PCA) with haplotype analyses subsequently performed for regions containing single‐nucleotide polymorphisms (SNPs) above the suggestive genome‐wide significance (GWS) threshold (P<1.0 × 10−5).

Results

One region containing 10 SNPs (Equus caballus chromosome [ECA] 7: 89,601,935–94,647,192) was above the suggestive GWS threshold. Two inferred haplotypes in this region demonstrated significant differences (P<0.001) between cases and controls, with the most frequent haplotype resulting in a significantly increased risk of DLC.

Main limitations

Small sample size as a result of stringent phenotyping protocols.

Conclusions

The current study highlights a candidate genomic region on ECA7 as potentially important with regard to the manifestation of DLC. Further exploration of this region and the genes included within it will bring veterinarians and researchers closer to fully understanding the biological mechanisms underlying DLC in horses.

Link to the publication

https://doi.org/10.1111/evj.13171

Reference

Velie, B. D., Smith, P. M., Fjordbakk, C. T., Solé, M., Jäderkvist Fegraeus, K., Rosengren, M. K., Røed, K. H., Ihler, C. F., Lindgren, G. and Strand, E. (2020), Exploring the genetics underpinning dynamic laryngeal collapse associated with poll flexion in Norwegian‐Swedish Coldblooded Trotter racehorses. Equine Vet J. 2020 Mar;52(2):174-180.