Methods available:
Bovine in vitro embryo production (from slaughterhouse material)
Porcine in vitro embryo production (from slaughterhouse material)
Feline in vitro embryo production (from discarded ovaries/testes after standard spaying in local veterinary clinics)
In vitro embryo production of ovines and other species can in some instances be set up
For all species development is followed:
Cumulus expansion after maturation
Cleavage after fertilization
Embryo development to blastocyst stage (blastocyst quality grading and blastocyst developmental stage)
Further work with blastocysts or earlier developmental stages:
Individual snap freezing in LN2 and storage in -80℃ for e.g. RNA-analysis (see below).
Individual fluorescent staining for confocal or fluorescence microscopy. Protocols currently available:
Neutral lipids (LipidTox or Nile Red)
Active mitochondria (Mitotrackers)
F-Actin (Alexafluor Phalloidin)
Nuclei (DAPI, Hoechst or Draq5)
Connected services (subject to availability from collaborators) includes:
Ovum Pic-Up (bovines)
RNA-expression analyses of embryos from -80℃ storage
Epigenetic analyses of embryos from -80℃ storage
Embryo transfer
Analyses of culture media
Hazard analysis
Please contact Ylva Sjunnesson for information regarding future projects, user’s fees and possibilities for collaboration!
Methods available:
Bovine in vitro embryo production (from slaughterhouse material)
Porcine in vitro embryo production (from slaughterhouse material)
Feline in vitro embryo production (from discarded ovaries/testes after standard spaying in local veterinary clinics)
In vitro embryo production of ovines and other species can in some instances be set up
For all species development is followed:
Cumulus expansion after maturation
Cleavage after fertilization
Embryo development to blastocyst stage (blastocyst quality grading and blastocyst developmental stage)