Ny sökning
PNS0151

Genome editing by CRISPR/Cas9 in theory and practice

Genome editing by CRISPR/Cas9 in theory and practice
The course provides the participants with a strong theoretical background in genome editing in form of lectures, seminars and open discussion. Furthermore, different applications of genome editing will be covered. This course will provide hands-on training in genome editing and cell engineering in plant and fungal species using genome editing with special emphasis on CRISPR/Cas9. Participants will learn to design CRISPR strategies using bioinformatics, generate gene knockouts/knock-ins, and validate targets using the most state of the art technologies. During the practical part students will design and attend an experiment within a given framework, analyse the results and present the data in a form, which would be required for a publication. The course consists of roughly equal theoretical and practical parts and will run on part time over a 4 week period.

Kursplan och övrig information


Kursplan

PNS0151 Genome editing by CRISPR/Cas9 in theory and practice, 5,0 Hp

Ämnen

Biologi

Utbildningens nivå

Forskarnivå

Språk

Engelska

Förkunskapskrav

The practical part of the course is primarily intended for PhD students within the SLU Graduate School Organism Biology, but will be open to all interested PhD students and researchers who want to learn how to edit genomes of plants/fungi using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required but experience in molecular biology is essential. All lectures will be carried out as open events, no registration is required to attend the theoretical part of the course.

Mål

After this course you should be able to design and implement CRISPR to manipulate your gene(s) of interest in, and validate the outcome of the procedure at the molecular level.

Innehåll

The course provides the participants with a strong theoretical background in genome editing in form of lectures, seminars and open discussion. Furthermore, different applications of genome editing will be covered. This course will provide hands-on training in genome editing and cell engineering in plant and fungal species using genome editing with special emphasis on CRISPR/Cas9. Participants will learn to design CRISPR strategies using bioinformatics, generate gene knockouts/knock-ins, and validate targets using the most state of the art technologies. During the practical part students will design and attend an experiment within a given framework, analyse the results and present the data in a form, which would be required for a publication. The course consists of roughly equal theoretical and practical parts and will run on part time over a 4 week period.

Ytterligare information

The course is organized by Panagiotis (Panos) Moschou on behalf of the SLU graduate school Organism Biology. Maximum 20 students per course occasion.

Ansvarig institution/motsvarande

Institutionen för växtbiologi

Kursfakta

Ämne: Biologi
Kurskod: PNS0151 Plats: Ortsoberoende Distanskurs: Ja Undervisningsspråk: Engelska Ansvarig institution: Institutionen för växtbiologi Studietakt: 100%