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Kursplan

PVS0152 Basic course for cell culture, 1,5 Hp

Ämnen

Biologi

Utbildningens nivå

Forskarnivå

Förkunskapskrav

Admitted to a postgraduate program in animal science, biology, veterinary medicine, or related subjects, or to a residency program in veterinary science (max 5 students per week)

Mål

The purpose of this course is to provide the Ph. D student and/or Master student the knowledge about i) primary cell culture; isolation, seeding and counting mammalian cells ii) methods of analysis for; cell survival, proliferation, apoptosis and iii) the basics of immune‐staining. Through practical laboratory work run by individual students it is intended to provide them the necessary know‐how and procedures to run cell culture under good practice rules insuring aseptic production and methods to evaluate cell survival. After completing the course the student shall be able to: 1. Prepare cells under an aseptic environment 2. Prepare of cell growth medium 3. Create the correct culturing environment 4. Operate an inverted light microscope for checking cells 5. Estimate percent confluence of a cell culture and how and when to change media 6. Determine the density of a cell culture using a hemocytometer 7. Dilute a cell culture for Sub‐culturing 8. Freeze cells in view of subsequent molecular analyzes 9. Evaluate Cell viability 10. Evaluate cell apoptosis 11. Stain cells to evaluate morphology

Innehåll

Time schedule and content of the course: Day 1, Morning: Lecture on cell biology, the basics of gene expression and epigenetics, ….(Patrice Humblot 1h+discussion), and Lecture on cell culture (Yongzhi Guo 1h+discussion) Afternoon: Autoclave small equipment (demonstration) Preparation of medium, thawing and seeding cells (demonstration and individual practice) Day 2, Detaching, counting and subculture of cells (demonstration and individual practice) Day 3, Morning: Collecting and freezing cells, create stocks (demonstration and individual practice) Afternoon: lecture (1h + discussion, Survival, proliferation, apoptosis) Day 4, Morning: Running apoptosis analysis (demonstration and individual practice) Afternoon: Hematoxylin staining (demonstration and individual practice) Day 5, Morning: Cell Proliferation test Afternoon: Theory examination

Ytterligare information

This course will be given in the framework of the "Cell for Life"

Infrastructure.

Ansvarig institution/motsvarande

Institutionen för kliniska vetenskaper