Cytological sample
Fine needle aspirate is administrated through injection needle (0,5-0,7 mm) and syringe (e.g. 5 ml). Local anaesthesia is not used. Insert the needle into the structure to be sampled and gently aspirate. Remove the syringe from the needle and take out the needle.Alternatively, several small smears can be made without aspiration. Place small amounts of the cell suspension on a microscope slide. Make smears immediately, preferably using both the standard blood smear technique and the "squash" technique. Allow to air dry and send the smears as they are. Use a microscope slide with a writing surface and mark with a regular pencil. Write the animal's name and/or journal number and the type of sample material taken.
Bone marrow
To make an optimal assessment of bone marrow, the following are needed:
- peripheral blood
- blood smear
- bone marrow smear for cytological assessment
- bone marrow biopsy for histopathological examination.
The Clinical Pathology Laboratory, in collaboration with the Department of Pathology (SLU), offers a bone marrow package that includes all analyses.
Provide information about medical history, clinical findings and previous test results on the referral as this will facilitate interpretation. There are different techniques for taking bone marrow smears, such as the standard blood smear technique and the “squash technique”. It is best to ensure that there are bone marrow particles in the smears, as samples without particles are more difficult to interpret and sometimes non-diagnostic due to too few bone marrow cells.
Bone marrow biopsies in formalin can be sent in a separate envelope to prevent the cytological smears from being destroyed by formalin fumes.
Bronchoalveolar lavage (BAL)
Bronchoalveolar lavage (BAL) is handled after sampling in the following way:
Divide the liquid into three test tubes, optimally with 10 ml in each tube, but sample amounts down to 2 ml will work. Indicate on the tubes whether it is original liquid or with added serum.
- A portion is left without action in an empty plastic tube or, if the liquid is mixed with blood, in an EDTA tube. Do not use serum tubes with gel or clot activator.
- A portion is mixed with serum, as this helps to preserve the morphology of the cells. It is possible to use serum from other individuals or animal species. When mixing, add approximately 1 part serum to 4 parts sample.
- A portion is centrifuged at low speed, approximately 300 G, and then a smear is made on the bottom sediment.
The fluid should preferably be analyzed within 24 hours, but smears have an unlimited shelf life and attached smears can therefore be analyzed for cellular changes and cell morphology in cases where the shelf life of the fluids has been exceeded.
Transcellular fluids (synovial, peritoneal and pleural fluid, CSF)
Viscous and blood-mixed fluids are collected in EDTA tubes, but it is advisable to supplement with plastic tubes without additives. Thin, cell-poor fluids are preferably collected in plastic tubes without additives. Remember to include a smear of the fluid.