P000042, Analysis of Genomes, 7.5 Hp
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Syllabus

Finalized by: Fun- VH, 2023-04-18
Valid from : Second half-year 2023 (2023-07-01)

Level

Third cycle

Grading Scale

The grade requirements within the course grading system are set out in specific criteria. These criteria must be available by the course start at the latest.

Course language

English

Entry Requirements

Objectives

The course intends to provide advanced knowledge of theory and methods for analysis of eukaryotic genomes, including their organization and evolution. There is a focus on animal genomics, but theoretical and methodological aspects of the course are applicable on many different organisms.

On completion of the course, the student should be able to:

• navigate and utilise available genomic data,
• design PCR-primers and -probes, and optimize PCR,
• perform DNA sequencing and analyse obtained data,
• detect and analyse sequence variation,
• predict functional consequences of genomic variation,
• describe and explain state of the art and large-scale methods to analyse genetic variation (eg. whole genome sequencing), and gene expression analysis (eg. RNA-seq),
• apply and use basic molecular genetic laboratory and bioinformatics methods,
• efficiently and systematically plan a genomic laboratory project,
• independently search for, summarize, interpret, and critically assess scientific articles in genetics, molecular genetics, and genomics,
• integrate knowledge in molecular genetics, and laboratory methodology to solve complex problems of relevance for genome analysis,
• discuss how the content of the course is relevant to the student´s own research project.

Content

The course is based on lectures, group discussions, exercises, and wet laboratory. The contents build largely on animal genome research. Theoretical teaching is for the most part directly applicable also within for example human or plant genetics. Genome science is rapidly evolving, and the course is based on current state-of-the-art methodology and research.

A large part of the course involves wet laboratory training including exercises and a laboratory project.

Computer exercises and group discussions will cover:

• genome sequence variation,
• genome browsing,
• primer design,
• sequence analysis,
• high throughput sequencing methodology

The aim of the computer exercises is to give the students useful tools for basic genetic and genomic analyses. Therefore, the computer exercises use free and open-source software that the student can download and use on their own laptop. In addition to the written and the oral examination, compulsory components occur within eg. exercises, group assignments and laboratory sessions.

Examination Formats and Requirements for Passing the Course

Passed participation including oral presentations and written reports in compulsory course modules. Written and oral examination.

Responsible Department/Equivalent

Department of Animal Breeding and Genetics

Supplementary information

Other Information

The course is given jointly with the advanced course Genome Analysis (BK0002), 15 credits. The schedule is planned as part time with most of the wet laboratory in the end of the course. Compulsory exercises eg. group discussions, and computer exercises could, if needed, be offered as digital solutions, while the wet laboratory sessions must be attended on campus.