PLS0085, Common Microscopy Methods in Biological Research, 3.0 Hp
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Syllabus

Level

Third cycle

Grading Scale

The grade requirements within the course grading system are set out in specific criteria. These criteria must be available by the course start at the latest.

Course language

English

Entry Requirements

Objectives

Visualization and understanding of morphological and cellular structures is of importance in biological research as well as for applications such as bio-based materials. The aim of the course is to give a brief theoretical background to and hands-on training on common low and high-resolution microscopy methods. A short introduction and hands-on training of optical and electron microscopy as well as fluorescence and confocal laser scanning microscopy is included. Special emphasis is given on how to prepare plant material for microscopy, but the course is applicable for any type of biological material.

Learning outcomes On completion of the course, the student will

• have gained overview of common microscopy methods
• have understanding on sample preparation for microscopy
• have some experience on using different microscopes
• be able to plan for microscopy applications in their own projects

Content

Sample preparation for different types of microscopy will be discussed. Participants can use their own material to certain extent during training sessions. Case-studies highlight how microscopy methods can be applied in research. The course is completed with a round-table discussion where the participants discuss given case studies and present possible applications of microscopy in their own projects. The course is planned as a part of the series of Postgraduate Courses in the Life Sciences, and located partly in Lund University, Department of Biology, and partly at SLU campus Alnarp, Department of Plant Protection Biology.

Examination Formats and Requirements for Passing the Course

What is required to pass To pass the course, a PhD student has participated in the course including discussion of case studies and a short presentation related to one’s own project.

Responsible Department/Equivalent

Department of Plant Protection Biology

Supplementary information

Other Information

Pedagogical form Lectures, case studies, active participation in training sessions and discussions, and a presentation will prepare students for planning and applying microscopy methods in their future projects.

Timetable Preliminary: Day 1. Introduction, lecture and hands-on sample preparation. Day 2. Lecture electron microscopy and hands-on. Day 3. Lecture Confocal Laser Scanning Microscopy and hands-on. Day 4. Confocal microscopy hands-on. Preparation of presentations. Day 5. Demonstration. Presentations and a round-table discussion. Literature will be available beforehand.

What is required to pass To pass the course, a PhD student has participated in the course including discussion of case studies and a short presentation related to one’s own project.