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Stephanie Robert

Stephanie Robert
For all living organisms, the survival and reproductive success strongly depends on their ability to perceive and integrate both external and internal signals. Due to their sessile life style and in contrast to most animals, plants have developed an extensive flexibility allowing them to modify their development in order to cope with fast changing environments. Such flexibility has been made possible by a set of morphological adjustments, which regulate the growth of organs, such as leaves or roots. Developmental changes are often mediated by cell elongation, which is a very complex process in plants due to the presence of the cell wall, located outside the cell membrane. Cell wall provides support and protection to the plant cell and thus has to be fairly rigid. However, this compartment also needs to be flexible to allow cell elongation and general growth.The plant primary cell wall is composed of cellulose microfibrils (the main component), hemicelluloses, pectins and proteins (Figure 1).The interactions between these components, modulated by different enzymatic activities such as hydrolysis, transglycosylation and disruption of hydrogen bonds,are believed to increase elasticity, thus permitting cell elongation. Furthermore, synthesis and deposition of new material is subsequently required to sustain growth.As a key regulator of plant development, IAA modulates cell elongation via the establishment of an auxin gradient. One of the auxin properties is to stimulate cell elongation by induc- ing wall loosening (Ray et al., 1972).This effect is enhanced in presence of gibberellins, highlighting the importance of phy- tohormones in the adjustment of the cell wall. Many aspects of the regulation of cell elongation/plant growth remain elusive and by using Arabidopsis thaliana, for which a wide range of biologic and molecular tools have been developed, there are undoubtedly opportunities to increase our comprehension of plant development processes.


Current appointment

Since August 2010, Assistant professor, Swedish University of Agricultural Sciences,

Department of Forest Genetics and Plant Physiology, Umeå (Forskarassistent (08-

2010 to 08-2014)/ Forskare (09-2014 to present))

University and university college degrees and diplomas

2005: Doctoral degree

PhD, Plant Science, Paris XI University-Orsay, France

2001: Master degree, Paris-Saclay University, France

Competence as associate professor

2015: Docent

 Other education

Teaching in higher education; course level 1 and 2

Course in grading and assessment

Course design

Course in doctoral supervision


I have been teaching as an assistant since my time as a Ph. D student and then giving

lectures since 2002. I am involved in several different courses at SLU and Umeå


2002-2004: Practical biology courses at INAPG (Institut National Agronomique

Paris-Grignon, 15 hrs/year)

Practical biology courses at University of Versailles St Quentin (Master

degree level, 12 hrs/year).

2010: Cell Biology class (Intracellular compartments & transport; Control of gene

expression; 2 to 4 hrs/year)

Functional Genomics (Chemical Biology; 2 hrs/year)

Plant development (Chemical biology, 2 hrs/year; Cell polarity, 2 hrs/year)

Agrochemical course (2 hrs/year)

2014: “Basic Biology” from the “bioresource engineering master program” (8 to 10


I was also invited to perform some plant development and chemical biology courses

in Olomouc (Czech Republic) and Singapore.

Since the start of my period as an assistant professor, I have been involved in the

supervision of five PhD students (two for which I am currently the main supervisor)

and several master students. I have been in charge of project design and guidance in i)

the experimental strategy establishment, ii) their laboratory work, iii) the

identification of relevant literature and courses for their scientific development. These

activities have included regular meetings with the students to assess their progress and

a day-by-day guidance in the lab. Below is the detailed description of these

supervision activities.


cell biology, hormone signaling, Arabidopsis, endomebrane traffciking, chemical biology

Selected publications

Publications (ordered chronologically)

Sum of the Times Cited: 1262

Average Citations per item: 40, 71

h-index: 17


Refereed journals

*: authors contributed equally to the work, †: corresponding author,  [n] Citations excluding self citations (May 9th, 2016, Web of Knowledge)


1-Dejonghe W, Kuenen S, Mylle E, Vasileva M, Keech O, Viotti C, Swerts J, Fendrych M, Ortiz-Morea FA, Mishev K, Delang S, Scholl S, Zarza X, Heilmann M, Kourelis J, Kasprowicz J, Nguyen LSL, Drozdzecki A, Van Houtte I, Szatmári AM, Majda M, Baisa G, Bednarek S, Robert S, Audenaert D, Testerink C, Munnik T, Van Damme D, Heilmann I, Schumacher K, Winne J, Friml J, Verstreken P, Russinova E (2016). Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. Nature communication 8;7:11710. doi: 10.1038/ncomms11710.


2- Jiskrová E, Novák O, Pospíšilová H, Holubová K, Karády M, Galuszka P, Robert S, Frébort I (2016). Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots. N Biotechnol. pii: S1871-6784(16)00002-9. doi: 10.1016/j.nbt.2015.12.010 [n.a]


3-Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml  J (2015). Osmotic pressure regulates the balance between exocytosis and clathrin-mediated endocytosis in Arabidopsis thaliana. Molecular Plant, pii: S1674-2052(15)00175-6. [2]


★4-Doyle SM, Haeger A, Vain T, Rigal A, Viotti C, Langowska M, Ma Q, Friml J, Raikhel NV, Hicks GR, Robert S†. (2015). An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. Proc Natl Acad Sci U S A., doi/10.1073/pnas.1424856112 [4]


5-Vain T, Crowell EF, Timpano H, Biot E, Desprez T, Mansoori N, Trindade LM, Pagant S, Robert S, Höfte H, Gonneau M, Vernhettes S (2014). The cellulase KORRIGAN is part of the Cellulose Synthase Complex. Plant physiology 165:1521-1532 [27]


6-Paudyal R, Jamaluddin A, Warren JP, Doyle SM, Robert S, Warriner SL and Baker A (2014). Trafficking modulator TENin1 inhibits endocytosis, causes endomembrane protein accumulation at the pre-vacuolar compartment and impairs gravitropic response in Arabidopsis thaliana. Biochemical Journal. 460: 177-85 [6]


7-Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C. (2013). ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis. The Plant Journal. 76:811-24 [10]


★8-Boutté Y, Jonsson K, McFarlane HE, Johnson E, Gendre D, Swarup R, Friml J, Samuels L, Robert S, Bhalerao RP. (2013). ECHIDNA-mediated post-Golgi trafficking of auxin carriers for differential cell elongation. Proc Natl Acad Sci U S A. 110:16259-64 [15]


9-Simon S, Kubeš M, Baster P, Robert S, Dobrev P, Friml J, Petrášek J, Zažímalová E. (2013). Defining selectivity of processes along the auxin response chain: a study using auxin analogues. New Phytologist. 200:1034-1048 [12]


10-Moschou P, Smertenko A, Minina E, Fukada K, Savenkov E, Robert S, Hussey P, Bozhkova P. (2013). The caspase-related protease separase (EXTRA SPINDLE POLES) regulates cell polarity and cytokinesis in Arabidopsis. The Plant Cell. 25 :2171-2186. [8]


11-Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru MI, De Rycke R, Robert S, Kakimoto T, Friml J. (2013). Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis. Plos Genetics, e1003540. doi: 10.1371/journal.pgen.1003540. [18]


12-Yu H, Karampelias M, Robert S, Peer W, Swarup R, Ye S, Ge L, Cohen J, Murphy A, Friml J, Estelle M. (2013). ROOT UVB SENSITIVE 1/WEAK AUXIN RESPONSE 3 is essential for polar auxin transport in Arabidopsis. Plant Physiology, 162: 965-76 [6]


    13-Baster P, Robert S, Kleine-Vehn J, Vanneste S, Kania, U, Grunewal W, De Rybel B, Beeckman T and Friml J (2012). Dual regulation of PIN vacuolar trafficking and auxin fluxes by differential auxin levels during root gravitropism. Embo Journal, 32:260-74. [34]


    14-Chen X, Naramoto, S, Robert S, Tejos R, Löfke C, Lin D, Yang Z, Friml J (2012). ABP1 and ROP6 GTPase signaling regulate clathrin-mediated endocytosis in Arabidopsis roots. Current Biology, 22:1326-32. [75]


15-Kleine-Vehn J, Wabnik K, Martinière A, Łangowski L, Willig K, Naramoto S, Leitner J, Tanaka H, Jakobs S, Robert S, Luschnig C, Govaerts W, Hell S, Runions J, Friml J (2011). Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at plasma membrane. Molecular System Biology, 7:540. doi: 10.1038/msb.2011.72. [62]


    ★16-Drakakaki G *, Robert S *, Szátmari A-M *, Brown M, Nagawa S, Van Damme D, Leonard M, Yang Z, Girke T, Schmid S, Russinova E, Friml J, Raikhel N, Hicks G (2011). Clusters of bioactive compounds target dynamic endomembrane networks in vivo. Proc Natl Acad Sci. USA, 108:17850-17855. [42]


    17-Barberon M*, Zelazny E*, Robert S, Conéjéro G, Curie C, Friml J, Vert G (2011). Monoubiquitin-dependent endocytosis of the IRON-REGULATED TRANORTER 1 (IRT1) transporter controls iron uptake in plants. Proc Natl Acad Sci. USA, 108: E450-8. [116]


    18-Kitakura S*, Vanneste S*, Robert S, Lofke C, Teichmann T, Tanaka H, Friml J (2011). Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis. The Plant Cell, 23: 1920–1931. [93]


    19-Naramoto S, Kleine-Vehn J, Robert S, Fujimoto M, Dainobu T, Paciorek T, Ueda T, Nakano A, Van Montagu MC, Fukuda H, Friml J (2010). ADP-ribosylation factor machinery mediates endocytosis in plant cells. Proc Natl Acad Sci. USA, 107: 21890–21895. [49]


    ★20-Robert S*, Kleine-Vehn J*, Barbez E, Sauer M, Paciorek T, Baster P, Vanneste S, Zhang J, Simon S, Covanova M, Hayashi H, Dhonukshe P, Yang Z, Bednarek S, Jones A, Luschnig C, Aniento F, Zazımalova E, and Friml J (2010). ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell, 143:111-121. [200]


       21-Ge L, Peer W, Robert S, Swarup R, Ye S, Prigge M, Cohen JD, Friml J, Murphy A, Tang D, Estelle M (2010). Arabidopsis ROOT UVB SENSITIVE2/WEAK AUXIN RESPONSE1 is required for polar auxin transport. The Plant Cell, 22: 1749–1761. [14]


       22-Drakakaki G, Robert S, Raikhel NV, Hicks GR (2009). Chemical dissection of endosomal pathways. Plant Signaling and Behavior, 4:1-6. [23]


    ★23-Robert S, Chary N, Drakakaki G, Yang Z, Raikhel N, Hicks G (2008). Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1. Proc Natl Acad Sci. USA, 105:8464-8469. [129]


    24-Robert S, Zouhar J, Carter C, Raikhel N (2007). Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts. Nature Protocols, 2: 259-262. [25]


       25-Sanmartín M, Ordóñez A, Sohn E-J, Robert S, Sánchez-Serrano J-J, Surpin M, Raikhel N, Rojo E (2007). Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis. Proc Natl Acad Sci. USA, 10:3645-3650. [74]


       26-Drakakaki G, Zabotina O, Delgado I, Robert S, Keegstra K, Raikhel N (2006). Arabidopsis RGP1 and RGP2 are essential for pollen development. Plant Physiology, 142:1480-1492. [55]


    27-Robert S, Bichet A, Grandjean O, Kierskowski D, Satiat-Jeunmaitre B, Pelletier S, Hauser M-T, Höfte H, Vernhettes S (2005). An Arabidopsis endo-1,4-β-D-glucanase involved in cellulose synthesis undergoes regulated cellular cycling. The Plant Cell, 17: 3378-3389. [77]


       28-Robert S, Mouille G, Höfte H (2004). The mechanism and regulation of cellulose synthesis in primary wall: lessons from primary cell wall mutants. Cellulose, 11: 351-364. [43]


Peer-reviewed reviews

*: authors contributed equally to the work, †: corresponding author,  [n] Citations excluding self citations (Web of Knowledge)


1-Doyle SM, Vain T, and Robert S†.  (2015) Small molecules unravel complex interplay between auxin biology and endomembrane trafficking. Journal of Experimental Botany, 66:4971-4982 [1]


2-Rigal A, Ma Q, Robert S† (2014). Unraveling plant hormone signaling through the use of small molecules. Frontier in Plant Science. doi: 10.3389/fpls.2014.00373 [5]


3-Ma Q, Robert S† (2014). Auxin biology revealed by small molecules. Physiologia Plantarum, 151: 25-42 [9]


4-Sauer M*, Robert S*, Kleine-Vehn J* (2013). Auxin: simply complicated. Darwin reviews. Journal of Experimental Botany, 64:2565-2577 [48]



Book chapters

*: authors contributed equally to the work, †: corresponding author,  [n] Citations excluding self citations (Web of Knowledge)


Co-editor of Plant Chemical Biology, Methods and Protocols-Methods in Molecular Biology 1056-Springer Protocols -Humana Press-Editors Stéphanie Robert Glenn R Hicks (2014)


1-Rigal A, Doyle S, Robert S† (2015) Live-cell imaging of FM4-64 as a tool for tracing the endocytic pathways in Arabidopsis root cells. Editors: Jose M. Estevez. Plant Cell Growth and Expansion - Methods and Protocols. 1242:93-103. doi: 10.1007/978-1-4939-1902-4_9. [n.a.]


2-Doyle S, Robert S † (2014). Using mutant studies combined with chemical genetics. Editors: Hicks G and Robert S. Publisher: Humana MiMB. Methods Mol Biol. 2014;1056:51-62. [n.a.]


3-Haeger,A, Langowska M, Robert S † (2013). The use of chemical biology to study plant cellular processes–subcellular trafficking. Editors: Overvoorde PJ and Audenaert D. Publisher: John Wiley & Sons, New Jersey. [n.a.]


4-Robert S, Raikhel NV, Hicks GR (2009) Powerful partners: Arabidopsis and chemical genomics. The Arabidopsis Book. Rockville, MD: American Society of Plant Biologists; 2009. p 1-16. [n.a.]