Microscopic image of a mussel shell showing the distribution of the elements strontium and manganese.
Brachiopod fossils analyzed using X-ray fluorescence (XRF) technology. The two different colors show the distribution of the elements strontium and manganese.

Micro-XRF for advanced chemical analyses

Page reviewed:  03/11/2025

Using X-ray Fluorescence (XRF) technology, we offer advanced analysis of the chemical composition of biological and geological materials – cost-effective and without damaging the samples.

The technology that makes the invisible visible – this is XRF

X-ray Fluorescence is a method used to determine the chemical composition of a material. Micro-XRF can focus the X-ray beam down to the micrometer level, allowing detailed mapping of elemental variations within very small areas of a sample. Because micro-XRF is non-destructive, sensitive and unique materials can be analyzed without physical alteration.

A resource for the entire research community

The potential of micro-XRF extends far beyond fish research. The instrument can be used across a wide range of research fields – from ecology and environmental analysis to archaeology, geology, and veterinary medicine.

How micro-XRF works – from sample to result

The sample is placed in a holder inside the instrument. The X-ray beam is focused, and an integrated camera captures an image of the sample, where the area to be mapped is marked. The analysis time depends on the desired resolution and the detection time per analysis point. After the analysis, different elemental layers can be visualized in colors of choice to illustrate the chemical composition.

Quick facts

Technology: Non-destructive micro-X-ray fluorescence, where X-rays cause atoms in the sample to emit characteristic fluorescence revealing the elemental composition.

Instrument: Bruker M4 Tornado.

Detection: Elements from sodium (Na) and heavier can be detected, but not lighter elements such as carbon, nitrogen, and oxygen.

Sample size: Maximum 330 mm × 170 mm and up to 7 kg.

Types of analyses:

  • Mapping of chemical composition (max 190 × 160 mm²).
  • Quantitative measurement of elemental concentrations along a line transect or at a specific point. Analyses can be performed in vacuum or air.

Sample materials:

  • Biological samples such as tissue samples, fish otoliths, plants, fossils, teeth, and mussel shells.
  • Geological samples such as minerals, soil, and sediments.
  • Construction materials like concrete and cement.
  • Artworks such as oil paintings and historical photographs.
  • Various metals in circuit boards.
  • Surface coatings.

Applications: fish migration, environmental tracing, archaeology, material characterization, and more.

Sample preparation: None or minimal preparation is required for flat/even-thickness samples. Preparation of cross-sections (cut or polished) can be provided for other sample types.

  • High sensitivity for trace elements
  • High spatial resolution (~20 μm)
  • Short analysis time (often within a day)
  • Flat daily rate: 6,500 SEK
  • Hourly rate (analyst): 1,500 SEK

Example 1: Mapping to identify eel otoliths chemically marked with strontium

  • 20 samples: 8,000 SEK
  • 40 samples: 12,000 SEK

Example 2: Line transect to identify eel otoliths chemically marked with strontium

  • 175 SEK per otolith
  • 10 samples: 8,250 SEK
  • 20 samples: 10,000 SEK

Example 3: Mapping of otoliths to identify fish migration between freshwater and brackish water

  • 20 samples: 8,000 SEK
  • 40 samples: 12,000 SEK

Example 4: Test analysis of a new sample type

9,000 SEK

Currently, there are no specific terms or conditions for the use of the micro-XRF instrument.

Image examples

Questions and requests 

Do you have questions or wish to use the micro-XRF instrument? Please contact Yvette Heimbrand.

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