Madhab Kumar Sen

I study how Arabidopsis thaliana rewires RNA splicing and gene regulatory networks to survive temperature stress. My research combines molecular genetics, genomics, and CRISPR–dCas9-based gene activation to switch on multiple endogenous genes from a single transgene, enabling pathway-level control beyond traditional single-gene approaches. Through this work, I aim to uncover how plants build temperature resilience and translate these insights into climate-resilient crop engineering.

I am a molecular plant biologist studying how RNA splicing and gene regulatory networks shape temperature adaptation in Arabidopsis thaliana. My current research focuses on alternative pre-mRNA splicing, spliceosome-associated protein complexes, and CRISPR–dCas9-based multiplex gene activation. I am establishing an innovative platform that enables the simultaneous activation of multiple endogenous plant genes using a single transgene, thereby facilitating pathway-level regulation and overcoming limitations of traditional single-gene approaches. By integrating molecular genetics, genomics, and plant stress biology, I aim to uncover fundamental mechanisms of plant temperature resilience and develop new strategies for climate-resilient crop engineering.

[2019 - 2022]: Ph.D. in Plant Sciences, Czech University of Life Sciences Prague (CZU), Czech Republic.

[2016 - 2018]: Master of Science (M.Sc.) in Agricultural Biotechnology, Ramakrishna Mission Vivekananda Education and Research Institute, India.

[2013 - 2016]: Bachelor of Science (B.Sc.) in Molecular Biology and Biotechnology, Kanchrapara College (affiliated under Kalyani University), India.

[June 2025 – Present]: Postdoctoral researcher (RNA splicing biology), Swedish University of Agricultural Sciences (SLU).

[Oct 2022 – May 2025]: Postdoctoral researcher (Plant Biotechnology), Czech University of Life Sciences Prague (CZU).

[Jan 2024 – May 2025]: Team member (Molecular biologist), Czech University of Life Sciences Prague (CZU).

[Jan 2022 – Sept 2022]: Student research assistant (Molecular biologist), Czech University of Life Sciences Prague (CZU).

[July 2018 – Sept 2018]: Junior research assistant (CRISPR-Cas9 project), National Institute of Pharmaceutical Education and Research, Kolkata. Project title: "Exploitation of Paired Nickases to enhance targeted knock-in: A modification of CRISPR Cas system".

[27th Jan – 28th Apr 2026]: Teaching in Higher Education, basic course (3 weeks).