Improved tapeworm diagnostics with LAMP PCR

Equine tapeworms: a diagnostic challenge

Tapeworms are intestinal parasites of grazing horses. The most common species, Anoplocephala perfoliata, can cause intestinal disease in some cases colic. Reliable detection of tapeworms is important for deciding when to treat the horse, but the diagnosis can be a challenge. Tapeworm eggs are released irregularly in feces, so infections can be missed when examining samples under a microscope, especially when there are few prasites. Improved diagnostics can help veterinarians and horse owners to make the right decision when to treat the horse and reduce unnecessary deworming.

About the project

This project aims to develop and validate a rapid, reliable and affordable DNA-based test for equine tapeworms, focusing on Anoplocephala perfoliata. The test uses loop-mediated isothermal amplification (LAMP) to detect parasite DNA in horse feces.

During the project period (2026–2028), we will develop and optimize a LAMP assay and evaluate how the samples should be taken for optimal diagnosis results. These results will be compared with the flotation method used at the Swedish Veterinary Agency (SVA). We will assess diagnostic performance, analysis time and cost. The long-term goal is to provide diagnostic laboratories with a tool for accurate diagnosis that is the base for targeted treatment decisions, improved horse welfare and sustainable parasite control.

What is LAMP?

LAMP, short for loop-mediated isothermal amplification, is a molecular technique that produces many copies of a specific DNA sequence, making small amounts of parasite DNA detectable. LAMP works under another principle compared to conventional PCR: it operates at a constant temperature and does not require a thermal cycler. Normally, the amplification step takes 30–60 minutes after sample preparation. The results can be detected directly after the amplification step, either through fluorescence or a color change in the tube. Since it only requires simple equipment, it has great potential for rapid, accessible and cost-effective testing. This project will transform these advantages into routine diagnosis of equine tapeworm infections.

Funding

This project is funded by the Swedish-Norwegian foundation for equine research.