NextGenHorse: Using Next-Generation Sequencing to Decode Equine Shape and Locomotion

NextGenHorse is an interdisciplinary project aimed at uncovering the genetic foundations of horse body shape and movement. Although conformation plays a crucial role in performance, health, and locomotor ability, it has traditionally been assessed through subjective visual evaluation. By combining whole-genome sequencing (WGS) with state-of-the-art 3D digital phenotyping, this project introduces an objective, data-driven approach to understanding equine morphology.

Using advanced tools such as the VAREN 3D anatomical model and markerless motion-capture systems, we will generate highly detailed body-shape and locomotion data from horses across multiple breeds. These phenotypes will be integrated with high-resolution genomic data to identify genetic variants that influence conformation, gait, and overall biomechanical function.

NextGenHorse also explores cutting-edge analytical methods, including Genomic Informational Field Theory (GIFT) and multimodal genomic large language models (LLMs), to link complex genomic patterns with fine-scale morphological traits. Ultimately, the project aims to determine whether a horse’s body shape can be predicted directly from its genome.

By merging innovative genomics, computational anatomy, and equine biomechanics, NextGenHorse will advance our understanding of genotype-phenotype relationships in horses and support future improvements in breeding, welfare, and performance evaluation.