CV-sida

Johan Lundqvist

Johan Lundqvist

Forskning

1. Toxicity pathways – a novel strategy in water quality assessment

Principal investigator: Johan Lundqvist

Performed in collaboration with: Uppsala University (SciLifeLab), The Helmholtz Centre for Environmental Research, and SLU Department of Aquatic Sciences and Assessment.

This project aims to develop a system for testing of water quality, based on the principle of toxicity pathways. Such system to detect environmental pollutants in water would be of great benefit for the environmental monitoring and the evaluation of the national environmental quality objectives, by enabling the measurement of relevant toxic potential in a large number of water samples. Further, this effect-based system would make it possible to evaluate the effect of actions taken to reduce pollution and to assess mixture effects of environmental pollutants and effects of unknown chemicals. The methodology will allow analysis of a large number of samples to a moderate cost. The test system will be based on cultured cells of human and fish origin. The cells will be transfected with DNA-constructs that are designed to respond to molecular key event in different toxicity pathways. These assays will allow us to investigate the effects of chemicals and water samples on a wide range of toxicity mechanisms. The main deliverables from this project will be increased knowledge on the toxicity pathway in vitro assays that are needed to detect the environmental pollutants that are of greatest importance in a Nordic perspective and the establishment of a panel of in vitro assays that can be used to assess the total toxicity of water samples. This will allow a fast and cost effective new way of monitoring four of the Swedish national environmental quality objectives.

Financial support: Formas (grant no 2014-1435), Konung Carl XVI Gustafs 50-årsfond för vetenskap, teknik och miljö, and the Royal Swedish Academy of Agriculture and Forestry.

2. The use of toxicity pathways to reduce and replace in vivo studies in fish

Principal investigator: Johan Lundqvist

This project aims to develop a system for animal-free testing of fish toxicity, based on the principle of toxicity pathways. European regulations require that a large number of chemicals should undergo assessment of aquatic toxicity, which often include animal experiments. In 2005, a total of more than 1.7 million fishes were used for experimental purposes in the

European Union. Assessment of the fish toxicity in toxicity pathway-based cell culture models instead of in animal experiments, will allow analysis of a large number of chemicals to a moderate cost and will significantly reduce the number of animals used for aquatic toxicity assessment of chemicals. The European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) has recently proposed development of cell cultured based assays as a key strategy to reduce and replace the use of fish in toxicity testing. The test system will be based on cultured cells of fish and human origin. The cells will be transfected with DNA-constructs that are designed to respond to molecular key event in different toxicity pathways. These assays will allow us to investigate the effects of chemicals on a wide range of toxicity mechanisms. The main deliverables from this project will be increased knowledge on the toxicity pathway in vitro assays that are needed for initial screening of fish toxicity and a publicly accessible panel of in vitro assays that can be used to assess the aquatic toxicity in a wide range of biological systems.

Financial support: Vetenskapsrådet (grant no 2015-03404).

Bakgrund

Doctoral degree (2011) in pharmaceutical biochemistry from Uppsala University, with a thesis entitled Enzymatic Regulation of Steroidogenesis and Nuclear Receptor Activation - Special Focus on Vitamin D and Sex Hormones (link to thesis).

Postdoctoral experience from the Danish Cancer Society Research Center and Stanford University in the project Vitamin D and breast cancer – molecular mechanisms (2011-2013).

Postdoctoral experience from the Swedish University of Agricultural Sciences in the project Integrated chemical and toxicological methods for early detection of hazardous chemicals in drinking water – SafeDrink (2013-2015)

Awards and grants

Research grants from Formas and the Royal Swedish Academy of Agriculture and Forestry for the project "Toxicity pathways – a novel strategy in water quality assessment".

Research grant from Vetenskapsrådet for the project "Toxicity pathways – a novel strategy to reduce and replace in vivo studies in fish".

Stipend from the royal trust Stiftelsen Konung Carl XVI Gustafs 50-årsfond för vetenskap, teknik och miljö (2014)

F1000 Poster Award at the 16th Workshop on Vitamin D (2013)

Young Investigator Award at the 15th Workshop on Vitamin D (2012)

SSMF Fellow, the Swedish Society for Medical Research (awarded 2013)

Postdoctoral stipends from the Swedish Academy of Pharmaceutical Sciences (awarded 2011 and 2012)

Professional memberships

European Society of Toxicology In Vitro (ESTIV)

Society of Toxicology (SOT)

Society of Environmental Toxicology and Chemistry (SETAC)


Kontaktinformation
Forskare vid Institutionen för biomedicin och veterinär folkhälsovetenskap (BVF); Enheten för farmakologi och toxikologi
Telefon: 018-671681
Postadress:
BVF, Avd för farmakologi och toxikologi, Box 7028
75007 UPPSALA
Besöksadress: Ulls väg 26, Uppsala