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Abhijeet Singh

Abhijeet Singh
My research at the department of Molecular Sciences deals with the development of novel modelling and surveillance method for the "Microbiological surveillance of Biogas Plants". The biogas plants operating at high ammonia conditions are largely prone to disturbance and failure. This occurs due to the non-equilibrium in the microbial consortia involved in different steps of the biogas generation especially acetogenesis and methanogenesis. There are few methods which are being used at the biogas plants which gives the information about the process disturbance. Unfortunately, these methods has limited importance as they analyse the resultant of the post disequilibrium state microbial community. We are aiming to develop a nucleic acid based early warning system, which can monitor the continuous changes in the microbial community structure and predict any possible process disturbance. The scope of this project extends the horizon of current biogas process monitoring and real-time surveillance. My research is developing on the solid grounds of cutting edge molecular analysis such as Next generation sequencing with Illumina MiSeq sequencing and PacBio sequencing of whole acetogenic microbial community and the syntrophic correlation with the methanogenic partners and Terminal restriction fragment length polymorphism (T-RFLP). An automated bioinformatic analysis pipeline for the high-throughput sequencing data analysis is under evaluation for the commercial scale biogas plants. The ultimate aim of my current research is to develop a method for the real time surveillance of biogas plants.

Teaching

BI1081 - Microbiology and Immunology 2016-2017

BI0856 - Microbiology 2017-2018 

Research

1. Bacterial community profiling in anaerobic digesters

2. Next-generation sequencing of the microbial community in anaerobic digesters

3. Creation and development of the database for the accurate bacterial identification of acetogenic bacterial population

 

Cooperation

1. Bayerische Landesanstalt für Landwirtschaft (Bavarian State Research Center for Agriculture), Hutthurm, Germany

2. Department of Civil and Environmental Engineering, Chalmers University of Technology, Göteborg, Sweden

3. Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark

Background

Plant Biology

Agriculture

Molecular phytopathology

Microfluidics

Microscopy

Selected publications

* Detection of novel syntrophic acetate-oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

* Effect of Start-Up Strategies and Electrode Materials on Carbon Dioxide Reduction on Biocathodes

* Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester


Contact

Postdoctor at the Department of Molecular Sciences; Mikrobiell bioteknologi
Postal address:
Institutionen för molekylära vetenskaper
Box 7015
750 07 Uppsala
Visiting address: Almas Allé 5, BioCentrum, Ultuna, Uppsala