PLS0030 Analytical Methods in Chemical Ecology, 6.0 Credits
Pass / Failed
The requirements for attaining different grades are described in the course assessment criteria which are contained in a supplement to the course syllabus. Current information on assessment criteria shall be made available at the start of the course.
The course is intended to PhD students in chemical ecology, analytical chemistry, ecology, biology or a related field, but it is also possible for postdocs and M.Sc. students with relevant training to take part.
After completion of the course, the student should:
• have a good knowledge and practical experience of methods for sampling organic compounds
• understand chromatography; both gas chromatography and liquid chromatography
• know how to choose an analytical column, and why …
• good knowledge different methods for identification of organic compounds
• know different methods for quantification, and how to perform reliability of quantitative analysis
• How to write a M&M for the chemical-analytical part of a publication – is also a part of the examination
Morning sessions are lectures, and afternoon sessions are laboratory practice. The lecture series follow the "Silverstein principle for rigorous identification of bioactive compounds", with the protocol "observe" (i.e. bioassay); "isolate" (i.e. collection of bioactive compounds); "separate" (i.e. chromatography); "identify" (i.e. chemical analysis), and all steps confirmed with repeated bioassays.
The lectures will describe different methods of collection or organic compounds, as aeration, SPE, SPME etc. General properties of chromatography and the specificity of different chromatographic methods will be highlighted. Modern identification methods in chemical ecology, and other organic analysis, will be described. This course will also give an introduction to interpretation of mass spectral.
During the afternoons, the course will include a practical part: volatile collection, sample preparation, GC-MS analysis, and evaluation, using the analytical instrumentation at Chemical Ecology, Plant Protection Biology, Swedish University of Agriculture (SLU), Alnarp.
Formats and requirements for examination
One aim of the course is to be able to write the chemical-analytical part of MATERIAL AND METHODS in a publication: each student shall one week before the end of the course submit the chemical-analytical part of the M&M of their written lab report to the organizer of the course: to be corrected by the course leader for pass on the course
The laboratory practice will be reported by each lab group (pair) the last day of the course, and the results and experiences will be discussed by the participants. The course will be evaluated anonymously by the participants.
The laboratory practice will run during several days, and the evaluation results should be reported both as a written lab report, to be mailed or handed in, and as an oral presentation (PowerPoint, etc).
Parallel with the laboratory practice, other methods, electrophysiology etc, used in Chemical Ecology will be introduced to the participants.
PhD students outside SLU and Postdocs pay a course fee of 4000 SEK.
Department of Plant Protection Biology