DNA barcoding of benthic diatoms
The aim of this project is to develop DNA metabarcoding of benthic diatoms for use in environmental assessment in Sweden
DNA barcoding of benthic diatoms can be used for ecological status classification of streams, and CEN standards are under development, but there are many questions that need to be investigated first.
One goal of this projekt is to achieve more complete and correct taxa lists for both traditional and molecular methods as another step for the routine usage of metabarcoding in the future.
In this way, it will be possible to use well-implemented diatom metrics for environmental assessment also with metabarcoding data.
Reference library, open access 21.9.2015: http://220.127.116.11/new_rsyst_alg/
Rimet et al. (2016). R-Syst::diatom: an open-access and curated barcode database for diatoms and freshwater monitoring. Database. DOI: 10.1093/database/baw016.
Groendahl Sophie, Kahlert Maria & Fink Patrick (2017). The best of both worlds: a combined approach for analyzing microalgal diversity via metabarcoding and morphology-based methods. PLoS ONE, (12) 12(2), DOI:10.1371/journal.pone.0172808
Vasselon, V., et al. (2017). "Application of high-throughput sequencing (HTS) metabarcoding to diatom biomonitoring: Do DNA extraction methods matter?" Freshwater Science 36(1): 162-177.