DNA barcoding of benthic diatoms
The aim of this project is to develop DNA metabarcoding of benthic diatoms for use in environmental assessment in Sweden.
DNA barcoding of benthic diatoms can be used for ecological status classification of streams, and CEN standards are under development, but there are many questions that need to be investigated first.
One goal of this projekt is to achieve more complete and correct taxa lists for both traditional and molecular methods as another step for the routine usage of metabarcoding in the future.
In this way, it will be possible to use well-implemented diatom metrics for environmental assessment also with metabarcoding data.
- INRA (FR), NIVA (NO), SYKE (FIN), Icelandic Institute of Freshwater Fisheries, The Royal Botanic Garden Edinburgh (UK).
- DNAqua-net - genomic tools for routine application of biomonitoring of European water bodies
- EDNA - national network to develop DNA methods for monitoring and assessment
- PhD student project "New methods improving freshwater conservation and management" Bonnie Bailet funded by Stiftelsen Oscar och Lili Lamms Minne
- Reference library, open access 21.9.2015.
- Rimet et al. (2016). R-Syst::diatom: an open-access and curated barcode database for diatoms and freshwater monitoring. Database. DOI: 10.1093/database/baw016.
- Groendahl Sophie, Kahlert Maria & Fink Patrick (2017). The best of both worlds: a combined approach for analyzing microalgal diversity via metabarcoding and morphology-based methods. PLoS ONE, (12) 12(2), DOI:10.1371/journal.pone.0172808
- Vasselon, V., et al. (2017). "Application of high-throughput sequencing (HTS) metabarcoding to diatom biomonitoring: Do DNA extraction methods matter?" Freshwater Science 36(1): 162-177.