Daniel Hofius' Lab - Plant-Pathogen Interactions

We have a general interest in understanding the molecular and genetic mechanisms by which plants interact with and defend themselves against pathogens.                 

We focus on two evolutionary conserved biological processes with fundamental roles in both organismal health and pathological circumstances: Autophagy (Greek for “self-eating”) representing a major cellular degradation and renewal system, and programmed cell death (PCD) referring to different routes of genetically controlled cell suicide. We particularly aim at deciphering their functions, regulation, and interplay in plant immunity and disease.

Our current research addresses the following themes:

• Role of autophagy in host immunity and pathogen-triggered cell death
• Pathogen modulation and pro-microbial functions of autophagy
• Crosstalk of autophagy with the ubiquitin-proteasome system
• Membrane trafficking and vacuole functions in autophagy and cell death
• Epigenetic regulation of plant immunity and cell death
• Manipulation of autophagy for improved pathogen and stress resistance

We apply state-of-the-art genetic, proteomic, biochemical and cell biological approaches, and work with different bacterial, viral, and oomycete/fungal pathogens mainly in the model plants Arabidopsis thaliana and Nicotiana benthamiana. In addition, we strive to translate our findings into crop plants like potato and tomato.

For more details of the lab activities, please visit our Research background and focus, Research projects, and Publications pages.

We are located at the Uppsala BioCenter and are part of the Linnéan Center for Plant Biology in Uppsala, the Nordic Autophagy Network/Society and the Transautophagy COST action. Our research is funded by the Swedish University of Agricultural Sciences (SLU), Knut-and-Alice Wallenberg (KAW) Foundation (Epifate project), Swedish Research Councils VR and FORMAS; Carl Tryggers Stiftelse (CTS), and the Federation of European Biochemical Societies (FEBS).

Group members

• Sheena Rasmussen, Postdoc email
• Jitka Ortmannová, Postdoc email
• Nirbhay Kushwaha, Postdoc email
• Dimitrios Kokoretsis, PhD student email
• Giulia Ghirardello, MSc student email

Former group members

• Anders Hafrén, Postdoc email (now group leader in this dept)
• Suayib Üstün, Postdoc 
• Ooi-kock Teh, Postdoc
• Ramesh Vetukuri, Postdoc
• Qinsong Liu, PhD student
• Anton Hochmuth, MSc student
• David Solbach, MSc student
• Dimitri Wauters, Erasmus student
• Selina Manuela Windecker, Erasmus student

Selected publications

Full publication list    PubMed    Google scholar

Shukla A, Hoffmann G, López-Gonzaléz S, Hofius D, Hafrén A (2021). Salicylic acid and the viral virulence factor 2b regulate the divergent roles of autophagy during Cucumber mosaic virus infection. Autophagy, Nov 5:1-13. doi: 10.1080/15548627.2021.1987674. Online ahead of print. PubMed

Dvořák Tomaštíková E, Hafrén A, Trejo-Arellano MS, Rasmussen SR, Santos-González J, Sako H, Köhler C, Hennig L, Hofius D (2021). Polycomb Repressive Complex 2 and KRYPTONITE regulate pathogen-induced programmed cell death in Arabidopsis. Plant Physiol. 185, 2003-2021  PubMed

Li Q, Serio R, Schofield A, Liu H, Rasmussen S, Hofius D, and Stone S (2020). Arabidopsis RING-type E3 ubiquitin ligase XBAT35.2 promotes proteasome-dependent degradation of ACD11 to attenuate abiotic stress tolerance. Plant J. 104, 1712-1723 PubMed

Kushwaha NK, Hafrén A, Hofius D (2019) Autophagy-virus interactions: from antiviral recognition to proviral manipulation. Mol. Plant Pathol. 20, 1211-1216  PubMed

Üstün S, Hafrén A, Liu Q, Marshall RS, Minina EA, Bozhkov P, Vierstra RD, Hofius D (2018) Bacteria exploit autophagy for proteasome degradation and enhanced virulence in plants. Plant Cell 30: 668–685  PubMed  More info

Liu Q, Vain T, Viotti C, Doyle SM, Tarkowská D, Novák O, Zipfel C, Sitbon F, Robert S, Hofius D (2018) Vacuole Integrity Maintained by DUF300 Proteins Is Required for Brassinosteroid Signaling Regulation. Mol. Plant 11, 553-567  PubMed

Hafrén A, Üstün S, Hochmuth A, Svenning S, Johansen T, Hofius D (2018) Turnip mosaic virus counteracts selective autophagy of the viral silencing suppressor HCpro. Plant Physiol. 175, 649-662  PubMed

Üstün S, Hafrén A, Hofius D (2017) Autophagy as a mediator of life and death in plants.  Curr. Opin. Plant Biol. 40, 122-130  PubMed

Hafrén A, Macia JL, Love AJ, Milner JJ, Drucker M, Hofius D (2017) Selective autophagy limits cauliflower mosaic virus infection by NBR1-mediated targeting of viral capsid protein and particles. Proc Natl Acad Sci U S A 114, E2026-E2035   Pubmed

Hofius D, Li L, Hafrén A, Coll NS (2017). Autophagy as an emerging arena for plant-pathogen interactions. Curr Opin Plant Biol. 38, 117-23   PubMed 

Mozgova I, Wildhaber T, Liu Q, Abou-Mansour E, L’Haridon F, Métraux J-P, Gruissem W, Hofius D, and Hennig L (2015). Chromatin assembly factor CAF-1 represses priming of plant defence response genes. Nature Plants Article No 15127   PubMed

Munch D*, Teh OK*, Malinovsky FG*, Liu Q, Vetukuri RR, El Kasmi F, Brodersen P, Hara-Nishimura I, Dangl JL, Petersen M, Mundy J, and Hofius D (2015). Retromer contributes to immunity-associated cell death in Arabidopsis. Plant Cell, 27, 463-79. (*co-first authors)

Minina EA, Bozhkov P, and Hofius D (2014). Autophagy as an initiator or executioner of programmed cell death in plants. Trends Plant Sci., 19, 692-7.

Munch D, Rodriguez E, Bressendorff S, Park OK, Hofius D, and Petersen M (2014). Autophagy deficiency leads to accumulation of ubiquitinated proteins, ER stress, and cell death in Arabidopsis. Autophagy 10, 1579-87.

Teh OK and Hofius D (2014). Membrane trafficking and autophagy in pathogen-triggered cell death and immunity. J. Exp. Bot. 65, 1297-312.

Hackenberg T, Juul T, Auzina A, Gwizdz S, Malolepszy A, van der Kelen K, Dam S, Bressendorff S, Lorenzen A, Roepstorff P, Lehmann Nielsen K, Jørgensen JE, Hofius D, van Breusegem F, Petersen M, and Andersen SU (2013). Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis. Plant Cell 25, 4616-26.

Hofius D, Schultz-Larsen T, Joensen J, Tsitsigiannis DI, Petersen NH, Jørgensen LB, Mattsson O, Jones JD, Mundy J, and Petersen M (2009). Autophagic components contribute to hypersensitive cell death in Arabidopsis. Cell 137, 773-783.

Visiting address:
SLU
BioCenter, Room A-471
Almas Allé 5
Uppsala 

Postal address: 
Department of Plant Biology 
SLU
PO Box 7080 
SE-750 07 Uppsala
Sweden