Safe ready to eat vegetables from farm to fork: The plant as a key for risk assessment and prevention of EHEC infections (acronym: Safe Salad)

Last changed: 25 October 2023

Fruit and vegetables are an essential component of a healthy diet. During the last decades, outbreaks of foodborne illnesses have increasingly been linked to fresh and/or minimally processed fruit and vegetables.

Shigatoxin producing E. coli, causing EHEC infections, was the causal agent for major outbreaks in Sweden and abroad with leafy vegetables and sprouts. This pathogen is particularly severe due to its severe symptoms and low infectious dosis. The major source for contamination is associated with animal or human feces. Contamination can occur in the entire farm-to-fork chain and failure during preharvest may not be counteracted by processing. This project “Safe Salad” applies a novel and unique approach, as it focuses on the vector, namely the plant and its microflora. It uses a comprehensive approach from farm to fork. Results on interactions between cropping and processing environment, the leaf microflora and the prevalence of EHEC are used as a basis for risk assessment and identification of risk factors before harvest and of vegetables at risk. The impact of nitrogen upply to the crop is highlighted. Explanatory models for adhesion on the leaf via the existing biofilm and the significance of the leaf microbiota for disease development is studied in animal trials. Obtained results are implemented in intervention studies.


The project is funded by: Formas

Duration of the project: 2012-2017

Project Leader: Beatrix Alsanius

Other contributors: Lars Mogren (coordinator),

SLU: Anna Karin Rosberg, Maria Karlsson, Julia Darlison, Emina Mulaosmanovic, Maria Grudén, Rahel Hartmann, Ivar Vågsholm, Sofia Boqvist, Karin Söderqvist

LTH: Åsa Håkansson, Elisabeth Uhlig, Göran Molin, Siv Ahrné,

Lunds univ, Med-fak: Diana Karpman, Bengt Jeppsson