I graduated as a veterinarian at the Faculty of Life Science in Denmark, 2008. During my first year after graduation I worked with small animals at veterinary clinics in Enköping and in Lund. From 2010 I have been employed as a PhD student and teacher at the department of Anatomy Physiology and Biochemistry at SLU.
Background to my research area
During the last decades there has been a growing concern for the increased frequency of reproductive disturbances seen in both animals and humans. Chemicals in the environment disrupting the action of reproductive hormones seems to be part of the explanation. To detect adverse effects on male reproduction, microscopic investigation of testicular tissue is considered to be the most sensitive tool. Subtle changes in cells in the testicles might lead to infertility. I am trying to find good robust methods to detect alterations in the male reproductive organs by using different staining techniques as well as image analyzes. I study both rat, a frequently used laboratory animal and wild animals, such as mink and polar bear, to find out how chemicals in our immediate environment affect male reproduction
My PhD project is analysis of male reproductive tract morphology for reproductive toxicology.
The aim of the first project was to find out how to best collect samples from wild animals to be able to separate damage due to inadequate sampling from damage due to disturbed reproduction. This resulted in a publication published in PLOS one (PLoS ONE 10(5): e0125139. doi:10.1371/journal.pone.0125139) with the title “Effect of Pre-Fixation Delay and Freezing on Mink Testicular Endpoints for Environmental Research” (Ellinor Spörndly-Nees, Elisabeth Ekstedt, Ulf Magnusson, Azadeh Fakhrzadeh, Cris L. Luengo Hendriks, Lena Holm)
Next project was to develop new methods to evaluate mink testicular tissue in environmental research. This is a joint project with the Center of Image Analysis at Uppsala University in order to develop methods using image analysis. The results were published in Journal of Environmental Toxicology and Chemistry (Environmental Toxicology and Chemistry, Vol. 9999, No. 9999, pp. 1–9, 2016. DOI: 10.1002/etc.3517) with the title “New computerized staging method to analyze mink testicular tissue in environmental research” (Azadeh Fakhrzadeh, Ellinor Spörndly-Nees, Elisabeth Ekstedt, Lena Holm, Cris L. Luengo Hendriks)
An ongoing collaboration with associate professor Monika Lind and Margareta Halin Lejonklou at Uppsala University and Julie Boberg at Technical University of Denmark where we investigate the effect of Bisphenol A on male reproduction on young and old rats that have been exposed to BPA during pregnancy and lactation.
Another ongoing project is to investigate how environmental chemicals effect the reproduction of male Polar bear. The Polar bear organs are collected in Greenland and the project is a collaboration with professor Christian Sonne from the Department of Bioscience - Artic Research Center, Roskilde, Denmark
Assoc prof Lena Holm SLU, PhD Elisabeth Ekstedt SLU, Professor Ulf Magnusson SLU, Professor Leif Norrgren SLU, Cris Luengo Flagship Bioscience, USA, Senior scientist Julie Boberg, DTU Denmark, professor Christian Sonne, Åhus University, Denmark
Publikationer i urval
Effect of Pre-Fixation Delay and Freezing on Mink Testicular Endpoints for Environmental Research.
Ellinor Spörndly-Nees, Elisabeth Ekstedt, Ulf Magnusson, Azadeh Fakhrzadeh, Cris L. Luengo Hendriks, Lena Holm
PLoS ONE 10(5): e0125139. doi:10.1371/journal.pone.0125139
New computerized staging method to analyze mink testicular tissue in environmental research.
Azadeh Fakhrzadeh, Ellinor Spörndly-Nees, Elisabeth Ekstedt, Lena Holm and Cris L. Luengo Hendriks
Environmental Toxicology and Chemistry, Vol. 9999, No. 9999, pp. 1–9, 2016. DOI: 10.1002/etc.3517